CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell

Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of consensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. OBJECTI...

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Detalles Bibliográficos
Autores principales: Flores-Nascimento, Mariane Cristina, Alessio, Aline Morandi, de Andrade Orsi, Fernanda Loureiro, Annichino-Bizzacchi, Joyce Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Hematologia e Hemoterapia 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382576/
https://www.ncbi.nlm.nih.gov/pubmed/25818819
http://dx.doi.org/10.1016/j.bjhh.2014.11.014
Descripción
Sumario:Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of consensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. OBJECTIVE: The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells. METHODS: Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies. RESULTS: In spite of the rarity of the events, they were detectable and presented similar inter-person numbers of circulating endothelial cells. CONCLUSION: The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.

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