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Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity
The somatosensory nervous system is critical for the organism's ability to respond to mechanical, thermal, and nociceptive stimuli. Somatosensory neurons are functionally and anatomically diverse but their molecular profiles are not well-defined. Here, we used transcriptional profiling to analy...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383053/ https://www.ncbi.nlm.nih.gov/pubmed/25525749 http://dx.doi.org/10.7554/eLife.04660 |
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author | Chiu, Isaac M Barrett, Lee B Williams, Erika K Strochlic, David E Lee, Seungkyu Weyer, Andy D Lou, Shan Bryman, Gregory S Roberson, David P Ghasemlou, Nader Piccoli, Cara Ahat, Ezgi Wang, Victor Cobos, Enrique J Stucky, Cheryl L Ma, Qiufu Liberles, Stephen D Woolf, Clifford J |
author_facet | Chiu, Isaac M Barrett, Lee B Williams, Erika K Strochlic, David E Lee, Seungkyu Weyer, Andy D Lou, Shan Bryman, Gregory S Roberson, David P Ghasemlou, Nader Piccoli, Cara Ahat, Ezgi Wang, Victor Cobos, Enrique J Stucky, Cheryl L Ma, Qiufu Liberles, Stephen D Woolf, Clifford J |
author_sort | Chiu, Isaac M |
collection | PubMed |
description | The somatosensory nervous system is critical for the organism's ability to respond to mechanical, thermal, and nociceptive stimuli. Somatosensory neurons are functionally and anatomically diverse but their molecular profiles are not well-defined. Here, we used transcriptional profiling to analyze the detailed molecular signatures of dorsal root ganglion (DRG) sensory neurons. We used two mouse reporter lines and surface IB4 labeling to purify three major non-overlapping classes of neurons: 1) IB4(+)SNS-Cre/TdTomato(+), 2) IB4(−)SNS-Cre/TdTomato(+), and 3) Parv-Cre/TdTomato(+) cells, encompassing the majority of nociceptive, pruriceptive, and proprioceptive neurons. These neurons displayed distinct expression patterns of ion channels, transcription factors, and GPCRs. Highly parallel qRT-PCR analysis of 334 single neurons selected by membership of the three populations demonstrated further diversity, with unbiased clustering analysis identifying six distinct subgroups. These data significantly increase our knowledge of the molecular identities of known DRG populations and uncover potentially novel subsets, revealing the complexity and diversity of those neurons underlying somatosensation. DOI: http://dx.doi.org/10.7554/eLife.04660.001 |
format | Online Article Text |
id | pubmed-4383053 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-43830532015-04-03 Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity Chiu, Isaac M Barrett, Lee B Williams, Erika K Strochlic, David E Lee, Seungkyu Weyer, Andy D Lou, Shan Bryman, Gregory S Roberson, David P Ghasemlou, Nader Piccoli, Cara Ahat, Ezgi Wang, Victor Cobos, Enrique J Stucky, Cheryl L Ma, Qiufu Liberles, Stephen D Woolf, Clifford J eLife Genomics and Evolutionary Biology The somatosensory nervous system is critical for the organism's ability to respond to mechanical, thermal, and nociceptive stimuli. Somatosensory neurons are functionally and anatomically diverse but their molecular profiles are not well-defined. Here, we used transcriptional profiling to analyze the detailed molecular signatures of dorsal root ganglion (DRG) sensory neurons. We used two mouse reporter lines and surface IB4 labeling to purify three major non-overlapping classes of neurons: 1) IB4(+)SNS-Cre/TdTomato(+), 2) IB4(−)SNS-Cre/TdTomato(+), and 3) Parv-Cre/TdTomato(+) cells, encompassing the majority of nociceptive, pruriceptive, and proprioceptive neurons. These neurons displayed distinct expression patterns of ion channels, transcription factors, and GPCRs. Highly parallel qRT-PCR analysis of 334 single neurons selected by membership of the three populations demonstrated further diversity, with unbiased clustering analysis identifying six distinct subgroups. These data significantly increase our knowledge of the molecular identities of known DRG populations and uncover potentially novel subsets, revealing the complexity and diversity of those neurons underlying somatosensation. DOI: http://dx.doi.org/10.7554/eLife.04660.001 eLife Sciences Publications, Ltd 2014-12-19 /pmc/articles/PMC4383053/ /pubmed/25525749 http://dx.doi.org/10.7554/eLife.04660 Text en © 2014, Chiu et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Genomics and Evolutionary Biology Chiu, Isaac M Barrett, Lee B Williams, Erika K Strochlic, David E Lee, Seungkyu Weyer, Andy D Lou, Shan Bryman, Gregory S Roberson, David P Ghasemlou, Nader Piccoli, Cara Ahat, Ezgi Wang, Victor Cobos, Enrique J Stucky, Cheryl L Ma, Qiufu Liberles, Stephen D Woolf, Clifford J Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity |
title | Transcriptional profiling at whole population and single cell levels
reveals somatosensory neuron molecular diversity |
title_full | Transcriptional profiling at whole population and single cell levels
reveals somatosensory neuron molecular diversity |
title_fullStr | Transcriptional profiling at whole population and single cell levels
reveals somatosensory neuron molecular diversity |
title_full_unstemmed | Transcriptional profiling at whole population and single cell levels
reveals somatosensory neuron molecular diversity |
title_short | Transcriptional profiling at whole population and single cell levels
reveals somatosensory neuron molecular diversity |
title_sort | transcriptional profiling at whole population and single cell levels
reveals somatosensory neuron molecular diversity |
topic | Genomics and Evolutionary Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383053/ https://www.ncbi.nlm.nih.gov/pubmed/25525749 http://dx.doi.org/10.7554/eLife.04660 |
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