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Absolute quantification of somatic DNA alterations in human cancer
We developed a computational method (ABSOLUTE) that infers tumor purity and malignant cell ploidy directly from analysis of somatic DNA alterations. ABSOLUTE can detect subclonal heterogeneity, somatic homozygosity, and calculate statistical sensitivity to detect specific aberrations. We used ABSOLU...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383288/ https://www.ncbi.nlm.nih.gov/pubmed/22544022 http://dx.doi.org/10.1038/nbt.2203 |
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author | Carter, Scott L. Cibulskis, Kristian Helman, Elena McKenna, Aaron Shen, Hui Zack, Travis Laird, Peter W. Onofrio, Robert C. Winckler, Wendy Weir, Barbara A. Beroukhim, Rameen Pellman, David Levine, Douglas A. Lander, Eric S. Meyerson, Matthew Getz, Gad |
author_facet | Carter, Scott L. Cibulskis, Kristian Helman, Elena McKenna, Aaron Shen, Hui Zack, Travis Laird, Peter W. Onofrio, Robert C. Winckler, Wendy Weir, Barbara A. Beroukhim, Rameen Pellman, David Levine, Douglas A. Lander, Eric S. Meyerson, Matthew Getz, Gad |
author_sort | Carter, Scott L. |
collection | PubMed |
description | We developed a computational method (ABSOLUTE) that infers tumor purity and malignant cell ploidy directly from analysis of somatic DNA alterations. ABSOLUTE can detect subclonal heterogeneity, somatic homozygosity, and calculate statistical sensitivity to detect specific aberrations. We used ABSOLUTE to analyze ovarian cancer data and identified pervasive subclonal somatic point mutations. In contrast, mutations occurring in key tumor suppressor genes, TP53 and NF1 were predominantly clonal and homozygous, as were mutations in a candidate tumor suppressor gene, CDK12. Analysis of absolute allelic copy-number profiles from 3,155 cancer specimens revealed that genome-doubling events are common in human cancer, and likely occur in already aneuploid cells. By correlating genome-doubling status with mutation data, we found that homozygous mutations in NF1 occurred predominantly in non-doubled samples. This finding suggests that genome doubling influences the pathways of tumor progression, with recessive inactivation being less common after genome doubling. |
format | Online Article Text |
id | pubmed-4383288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-43832882015-04-02 Absolute quantification of somatic DNA alterations in human cancer Carter, Scott L. Cibulskis, Kristian Helman, Elena McKenna, Aaron Shen, Hui Zack, Travis Laird, Peter W. Onofrio, Robert C. Winckler, Wendy Weir, Barbara A. Beroukhim, Rameen Pellman, David Levine, Douglas A. Lander, Eric S. Meyerson, Matthew Getz, Gad Nat Biotechnol Article We developed a computational method (ABSOLUTE) that infers tumor purity and malignant cell ploidy directly from analysis of somatic DNA alterations. ABSOLUTE can detect subclonal heterogeneity, somatic homozygosity, and calculate statistical sensitivity to detect specific aberrations. We used ABSOLUTE to analyze ovarian cancer data and identified pervasive subclonal somatic point mutations. In contrast, mutations occurring in key tumor suppressor genes, TP53 and NF1 were predominantly clonal and homozygous, as were mutations in a candidate tumor suppressor gene, CDK12. Analysis of absolute allelic copy-number profiles from 3,155 cancer specimens revealed that genome-doubling events are common in human cancer, and likely occur in already aneuploid cells. By correlating genome-doubling status with mutation data, we found that homozygous mutations in NF1 occurred predominantly in non-doubled samples. This finding suggests that genome doubling influences the pathways of tumor progression, with recessive inactivation being less common after genome doubling. 2012-05 /pmc/articles/PMC4383288/ /pubmed/22544022 http://dx.doi.org/10.1038/nbt.2203 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Carter, Scott L. Cibulskis, Kristian Helman, Elena McKenna, Aaron Shen, Hui Zack, Travis Laird, Peter W. Onofrio, Robert C. Winckler, Wendy Weir, Barbara A. Beroukhim, Rameen Pellman, David Levine, Douglas A. Lander, Eric S. Meyerson, Matthew Getz, Gad Absolute quantification of somatic DNA alterations in human cancer |
title | Absolute quantification of somatic DNA alterations in human cancer |
title_full | Absolute quantification of somatic DNA alterations in human cancer |
title_fullStr | Absolute quantification of somatic DNA alterations in human cancer |
title_full_unstemmed | Absolute quantification of somatic DNA alterations in human cancer |
title_short | Absolute quantification of somatic DNA alterations in human cancer |
title_sort | absolute quantification of somatic dna alterations in human cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383288/ https://www.ncbi.nlm.nih.gov/pubmed/22544022 http://dx.doi.org/10.1038/nbt.2203 |
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