Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens

Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides...

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Autores principales: THANASARASAKULPONG, Arunee, POOLPERM, Pichayanut, TANKAEW, Pallop, SAWADA, Takuo, STHITMATEE, Nattawooti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383778/
https://www.ncbi.nlm.nih.gov/pubmed/25650149
http://dx.doi.org/10.1292/jvms.14-0532
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author THANASARASAKULPONG, Arunee
POOLPERM, Pichayanut
TANKAEW, Pallop
SAWADA, Takuo
STHITMATEE, Nattawooti
author_facet THANASARASAKULPONG, Arunee
POOLPERM, Pichayanut
TANKAEW, Pallop
SAWADA, Takuo
STHITMATEE, Nattawooti
author_sort THANASARASAKULPONG, Arunee
collection PubMed
description Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB. Therefore, the vaccines formulations in the present study can be considered new intranasal vaccine formulations for fowl cholera in chickens.
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spelling pubmed-43837782015-04-06 Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens THANASARASAKULPONG, Arunee POOLPERM, Pichayanut TANKAEW, Pallop SAWADA, Takuo STHITMATEE, Nattawooti J Vet Med Sci Bacteriology Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB. Therefore, the vaccines formulations in the present study can be considered new intranasal vaccine formulations for fowl cholera in chickens. The Japanese Society of Veterinary Science 2014-12-20 2015-03 /pmc/articles/PMC4383778/ /pubmed/25650149 http://dx.doi.org/10.1292/jvms.14-0532 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Bacteriology
THANASARASAKULPONG, Arunee
POOLPERM, Pichayanut
TANKAEW, Pallop
SAWADA, Takuo
STHITMATEE, Nattawooti
Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens
title Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens
title_full Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens
title_fullStr Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens
title_full_unstemmed Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens
title_short Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens
title_sort protectivity conferred by immunization with intranasal recombinant outer membrane protein h from pasteurella multocida serovar a:1 in chickens
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4383778/
https://www.ncbi.nlm.nih.gov/pubmed/25650149
http://dx.doi.org/10.1292/jvms.14-0532
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