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Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana

The study aimed to investigate the aetiological agents and clinical presentations associated with acute lower respiratory infections (ALRI) among children under five years old at the Korle-Bu Teaching Hospital in Ghana. This was a cross-sectional study carried from February to December 2001. Nasopha...

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Autores principales: Adiku, Theophilus K., Asmah, Richard H., Rodrigues, Onike, Goka, Bamenla, Obodai, Evangeline, Adjei, Andrew A., Donkor, Eric S., Armah, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384070/
https://www.ncbi.nlm.nih.gov/pubmed/25629622
http://dx.doi.org/10.3390/pathogens4010022
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author Adiku, Theophilus K.
Asmah, Richard H.
Rodrigues, Onike
Goka, Bamenla
Obodai, Evangeline
Adjei, Andrew A.
Donkor, Eric S.
Armah, George
author_facet Adiku, Theophilus K.
Asmah, Richard H.
Rodrigues, Onike
Goka, Bamenla
Obodai, Evangeline
Adjei, Andrew A.
Donkor, Eric S.
Armah, George
author_sort Adiku, Theophilus K.
collection PubMed
description The study aimed to investigate the aetiological agents and clinical presentations associated with acute lower respiratory infections (ALRI) among children under five years old at the Korle-Bu Teaching Hospital in Ghana. This was a cross-sectional study carried from February to December 2001. Nasopharyngeal aspirates and venous blood specimens obtained from 108 children with features suggestive of ALRI, were cultured and the isolated bacterial organisms were identified biochemically. Nasopharyngeal aspirates were also tested for Respiratory Syncitial Virus (RSV) antigen using a commercial kit (Becton Dickinson Directigen RSV test kit). A multiplex reverse transcription-PCR (RT-PCR) was also used to detect and characterize RSV using extracted RNA. Socio-demographic and clinical data were also obtained from the study subjects. Bronchopneumonia (55.5%), bronchiolitis (25%), lobar pneumonia (10.2), non-specific ALRI (4.6%), TB, bronchitis and respiratory distress (0.67%) were diagnosed. The prevalence of septicaemia was 10% and bacteria isolated were Staphylococcus aureus, Streptococcus pneumoniae and enteric bacteria, including Salmonella spp., Enterobacter spp and Klebsiella spp, were isolated. Out of the 108 cases, 18% tested positive for RSV, with two cases having RSV as the only aetiological pathogen detected. The subtyping analysis of RSV strains by a multiplex RT-PCR showed that subgroups A and B circulated in the season of analysis.
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spelling pubmed-43840702015-05-18 Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana Adiku, Theophilus K. Asmah, Richard H. Rodrigues, Onike Goka, Bamenla Obodai, Evangeline Adjei, Andrew A. Donkor, Eric S. Armah, George Pathogens Article The study aimed to investigate the aetiological agents and clinical presentations associated with acute lower respiratory infections (ALRI) among children under five years old at the Korle-Bu Teaching Hospital in Ghana. This was a cross-sectional study carried from February to December 2001. Nasopharyngeal aspirates and venous blood specimens obtained from 108 children with features suggestive of ALRI, were cultured and the isolated bacterial organisms were identified biochemically. Nasopharyngeal aspirates were also tested for Respiratory Syncitial Virus (RSV) antigen using a commercial kit (Becton Dickinson Directigen RSV test kit). A multiplex reverse transcription-PCR (RT-PCR) was also used to detect and characterize RSV using extracted RNA. Socio-demographic and clinical data were also obtained from the study subjects. Bronchopneumonia (55.5%), bronchiolitis (25%), lobar pneumonia (10.2), non-specific ALRI (4.6%), TB, bronchitis and respiratory distress (0.67%) were diagnosed. The prevalence of septicaemia was 10% and bacteria isolated were Staphylococcus aureus, Streptococcus pneumoniae and enteric bacteria, including Salmonella spp., Enterobacter spp and Klebsiella spp, were isolated. Out of the 108 cases, 18% tested positive for RSV, with two cases having RSV as the only aetiological pathogen detected. The subtyping analysis of RSV strains by a multiplex RT-PCR showed that subgroups A and B circulated in the season of analysis. MDPI 2015-01-26 /pmc/articles/PMC4384070/ /pubmed/25629622 http://dx.doi.org/10.3390/pathogens4010022 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Adiku, Theophilus K.
Asmah, Richard H.
Rodrigues, Onike
Goka, Bamenla
Obodai, Evangeline
Adjei, Andrew A.
Donkor, Eric S.
Armah, George
Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana
title Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana
title_full Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana
title_fullStr Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana
title_full_unstemmed Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana
title_short Aetiology of Acute Lower Respiratory Infections among Children Under Five Years in Accra, Ghana
title_sort aetiology of acute lower respiratory infections among children under five years in accra, ghana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384070/
https://www.ncbi.nlm.nih.gov/pubmed/25629622
http://dx.doi.org/10.3390/pathogens4010022
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