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Exploring possible DNA structures in real-time polymerase kinetics using Pacific Biosciences sequencer data

BACKGROUND: Pausing of DNA polymerase can indicate the presence of a DNA structure that differs from the canonical double-helix. Here we detail a method to investigate how polymerase pausing in the Pacific Biosciences sequencer reads can be related to DNA sequences. The Pacific Biosciences sequencer...

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Detalles Bibliográficos
Autores principales:	Sawaya, Sterling, Boocock, James, Black, Michael A, Gemmell, Neil J
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4384361/ https://www.ncbi.nlm.nih.gov/pubmed/25626999 http://dx.doi.org/10.1186/s12859-014-0449-0

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