Structural organization of the dynein-dynactin complex bound to microtubules

Cytoplasmic dynein associates with dynactin to drive cargo movement on microtubules, but the structure of the dynein-dynactin complex is unknown. Using electron microscopy, we determined the organization of native bovine dynein, dynactin, and the dynein-dynactin-microtubule quaternary complex. In th...

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Autores principales: Chowdhury, Saikat, Ketcham, Stephanie A., Schroer, Trina A., Lander, Gabriel C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4385409/
https://www.ncbi.nlm.nih.gov/pubmed/25751425
http://dx.doi.org/10.1038/nsmb.2996
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author Chowdhury, Saikat
Ketcham, Stephanie A.
Schroer, Trina A.
Lander, Gabriel C.
author_facet Chowdhury, Saikat
Ketcham, Stephanie A.
Schroer, Trina A.
Lander, Gabriel C.
author_sort Chowdhury, Saikat
collection PubMed
description Cytoplasmic dynein associates with dynactin to drive cargo movement on microtubules, but the structure of the dynein-dynactin complex is unknown. Using electron microscopy, we determined the organization of native bovine dynein, dynactin, and the dynein-dynactin-microtubule quaternary complex. In the microtubule-bound complex, the dynein motor domains are positioned for processive unidirectional movement and the cargo binding domains of both dynein and dynactin are accessible.
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spelling pubmed-43854092015-10-01 Structural organization of the dynein-dynactin complex bound to microtubules Chowdhury, Saikat Ketcham, Stephanie A. Schroer, Trina A. Lander, Gabriel C. Nat Struct Mol Biol Article Cytoplasmic dynein associates with dynactin to drive cargo movement on microtubules, but the structure of the dynein-dynactin complex is unknown. Using electron microscopy, we determined the organization of native bovine dynein, dynactin, and the dynein-dynactin-microtubule quaternary complex. In the microtubule-bound complex, the dynein motor domains are positioned for processive unidirectional movement and the cargo binding domains of both dynein and dynactin are accessible. 2015-03-09 2015-04 /pmc/articles/PMC4385409/ /pubmed/25751425 http://dx.doi.org/10.1038/nsmb.2996 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chowdhury, Saikat
Ketcham, Stephanie A.
Schroer, Trina A.
Lander, Gabriel C.
Structural organization of the dynein-dynactin complex bound to microtubules
title Structural organization of the dynein-dynactin complex bound to microtubules
title_full Structural organization of the dynein-dynactin complex bound to microtubules
title_fullStr Structural organization of the dynein-dynactin complex bound to microtubules
title_full_unstemmed Structural organization of the dynein-dynactin complex bound to microtubules
title_short Structural organization of the dynein-dynactin complex bound to microtubules
title_sort structural organization of the dynein-dynactin complex bound to microtubules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4385409/
https://www.ncbi.nlm.nih.gov/pubmed/25751425
http://dx.doi.org/10.1038/nsmb.2996
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