Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius

A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecti...

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Autores principales: Narwal, Sunil Kumar, Saun, Nitin Kumar, Dogra, Priyanka, Chauhan, Ghanshyam, Gupta, Reena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4385648/
https://www.ncbi.nlm.nih.gov/pubmed/25874205
http://dx.doi.org/10.1155/2015/281934
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author Narwal, Sunil Kumar
Saun, Nitin Kumar
Dogra, Priyanka
Chauhan, Ghanshyam
Gupta, Reena
author_facet Narwal, Sunil Kumar
Saun, Nitin Kumar
Dogra, Priyanka
Chauhan, Ghanshyam
Gupta, Reena
author_sort Narwal, Sunil Kumar
collection PubMed
description A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography.
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spelling pubmed-43856482015-04-13 Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius Narwal, Sunil Kumar Saun, Nitin Kumar Dogra, Priyanka Chauhan, Ghanshyam Gupta, Reena Biomed Res Int Research Article A novel thermotolerant lipase from Bacillus aerius was immobilized on inexpensive silica gel matrix. The immobilized lipase was used for the synthesis of biodiesel using castor oil as a substrate in a solvent free system at 55°C under shaking in a chemical reactor. Several crucial parameters affecting biodiesel yield such as incubation time, temperature, substrate molar ratio, and amount of lipase were optimized. Under the optimized conditions, the highest biodiesel yield was up to 78.13%. The characterization of synthesized biodiesel was done through FTIR spectroscopy, (1)H NMR spectra, and gas chromatography. Hindawi Publishing Corporation 2015 2015-03-22 /pmc/articles/PMC4385648/ /pubmed/25874205 http://dx.doi.org/10.1155/2015/281934 Text en Copyright © 2015 Sunil Kumar Narwal et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Narwal, Sunil Kumar
Saun, Nitin Kumar
Dogra, Priyanka
Chauhan, Ghanshyam
Gupta, Reena
Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius
title Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius
title_full Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius
title_fullStr Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius
title_full_unstemmed Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius
title_short Production and Characterization of Biodiesel Using Nonedible Castor Oil by Immobilized Lipase from Bacillus aerius
title_sort production and characterization of biodiesel using nonedible castor oil by immobilized lipase from bacillus aerius
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4385648/
https://www.ncbi.nlm.nih.gov/pubmed/25874205
http://dx.doi.org/10.1155/2015/281934
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