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Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran
BACKGROUND: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore, the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386043/ https://www.ncbi.nlm.nih.gov/pubmed/25848389 |
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author | GHOLAMI, Shirzad KHANMOHAMMADI, Majid AHMADPOUR, Ehsan PAGHEH, Abdol Sattar KHADEM NAKHJIRI, Sara RAMAZANNIPOUR, Hamidreza SHAHBAZI, Abbas |
author_facet | GHOLAMI, Shirzad KHANMOHAMMADI, Majid AHMADPOUR, Ehsan PAGHEH, Abdol Sattar KHADEM NAKHJIRI, Sara RAMAZANNIPOUR, Hamidreza SHAHBAZI, Abbas |
author_sort | GHOLAMI, Shirzad |
collection | PubMed |
description | BACKGROUND: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore, the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted to hospitals of Mazandaran University of Medical Sciences by parasitological and molecular methods in Sari, Iran. METHODS: Stool samples were collected from 348 patients with gastroenteritis admitted to the hospitals of Medical University in the Sari and Ghaemshahr cities in Mazandaran Province, Northern Iran in 2010-2011. Oocysts of Cryptosporidium identified using Formalin-Ether concentration method and stained by Aacid-fast staining (AFS) and Auramine phenol fluorescence (APF). Genomic DAN extracted from microscopically positive samples and nested PCR -RFLP by using SSU rRNA that identifies of the species of cryptosporidium. RESULTS: In 348 patients with gastroenteritis, the most clinical symptoms were diarrhea, nausea, vomiting, dehydration, fever and weight loss. 2.3% (8 cases) of diarrheal samples tested by both microscopy and molecular methods were positive for the presence of cryptosporidium. Nested PCR products yielded unique bands of 846 bp, correspond to cryptosporidium. Species diagnosis carried out by digesting the secondary PCR product with SspI restriction enzyme, which noted 3 clearly bands of 449, 254, and 108 bp correspond to Cryptosporidium spp. CONCLUSION: The results of present study on Cryptosporidium spp. in this area can make a background data for control programs and further molecular analyses. Thus, further work needs to determine the origin of Cryptosporidium species in this area. |
format | Online Article Text |
id | pubmed-4386043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-43860432015-04-06 Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran GHOLAMI, Shirzad KHANMOHAMMADI, Majid AHMADPOUR, Ehsan PAGHEH, Abdol Sattar KHADEM NAKHJIRI, Sara RAMAZANNIPOUR, Hamidreza SHAHBAZI, Abbas Iran J Parasitol Short Communication BACKGROUND: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. Therefore, the aim of this study was to determine the occurrence of Cryptosporidium infection in patients with gastroenteritis admitted to hospitals of Mazandaran University of Medical Sciences by parasitological and molecular methods in Sari, Iran. METHODS: Stool samples were collected from 348 patients with gastroenteritis admitted to the hospitals of Medical University in the Sari and Ghaemshahr cities in Mazandaran Province, Northern Iran in 2010-2011. Oocysts of Cryptosporidium identified using Formalin-Ether concentration method and stained by Aacid-fast staining (AFS) and Auramine phenol fluorescence (APF). Genomic DAN extracted from microscopically positive samples and nested PCR -RFLP by using SSU rRNA that identifies of the species of cryptosporidium. RESULTS: In 348 patients with gastroenteritis, the most clinical symptoms were diarrhea, nausea, vomiting, dehydration, fever and weight loss. 2.3% (8 cases) of diarrheal samples tested by both microscopy and molecular methods were positive for the presence of cryptosporidium. Nested PCR products yielded unique bands of 846 bp, correspond to cryptosporidium. Species diagnosis carried out by digesting the secondary PCR product with SspI restriction enzyme, which noted 3 clearly bands of 449, 254, and 108 bp correspond to Cryptosporidium spp. CONCLUSION: The results of present study on Cryptosporidium spp. in this area can make a background data for control programs and further molecular analyses. Thus, further work needs to determine the origin of Cryptosporidium species in this area. Tehran University of Medical Sciences 2014 /pmc/articles/PMC4386043/ /pubmed/25848389 Text en Copyright: © Iranian Journal of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Short Communication GHOLAMI, Shirzad KHANMOHAMMADI, Majid AHMADPOUR, Ehsan PAGHEH, Abdol Sattar KHADEM NAKHJIRI, Sara RAMAZANNIPOUR, Hamidreza SHAHBAZI, Abbas Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title | Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_full | Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_fullStr | Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_full_unstemmed | Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_short | Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran |
title_sort | cryptosporidium infection in patients with gastroenteritis in sari, iran |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386043/ https://www.ncbi.nlm.nih.gov/pubmed/25848389 |
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