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Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah

BACKGROUND: Wild, synanthropic and domestic mammals act as hosts and/or reservoirs of several Leishmania spp. Studies on possible reservoirs of Leishmania in different areas are fundamental to understand host-parasite interactions and develop strategies for the surveillance and control of leishmania...

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Autores principales: Cardoso, Rebecca Martins, de Araújo, Nadjar Nitz Silva Lociks, Romero, Gustavo Adolfo Sierra, Souza, Thaís Tâmara Castro Minuzzi, Dietrich, Ana Gabriela, Mendes, Júnio Donizette, Reis, Marcelo Lima, Ferreira, Jônatas Barbosa Cavalcante, Hecht, Mariana Machado, Gurgel-Gonçalves, Rodrigo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386096/
https://www.ncbi.nlm.nih.gov/pubmed/25889365
http://dx.doi.org/10.1186/s13071-015-0780-y
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author Cardoso, Rebecca Martins
de Araújo, Nadjar Nitz Silva Lociks
Romero, Gustavo Adolfo Sierra
Souza, Thaís Tâmara Castro Minuzzi
Dietrich, Ana Gabriela
Mendes, Júnio Donizette
Reis, Marcelo Lima
Ferreira, Jônatas Barbosa Cavalcante
Hecht, Mariana Machado
Gurgel-Gonçalves, Rodrigo
author_facet Cardoso, Rebecca Martins
de Araújo, Nadjar Nitz Silva Lociks
Romero, Gustavo Adolfo Sierra
Souza, Thaís Tâmara Castro Minuzzi
Dietrich, Ana Gabriela
Mendes, Júnio Donizette
Reis, Marcelo Lima
Ferreira, Jônatas Barbosa Cavalcante
Hecht, Mariana Machado
Gurgel-Gonçalves, Rodrigo
author_sort Cardoso, Rebecca Martins
collection PubMed
description BACKGROUND: Wild, synanthropic and domestic mammals act as hosts and/or reservoirs of several Leishmania spp. Studies on possible reservoirs of Leishmania in different areas are fundamental to understand host-parasite interactions and develop strategies for the surveillance and control of leishmaniasis. In the present study, we evaluated the Leishmania spp. occurrence in mammals in two conservation units and their surroundings in Brasília, Federal District (FD), Brazil. METHODS: Small mammals were captured in Brasília National Park (BNP) and Contagem Biological Reserve (CBR) and dogs were sampled in residential areas in their vicinity. Skin and blood samples were evaluated by PCR using different molecular markers (D7 24Sα rRNA and rDNA ITS1). Leishmania species were identified by sequencing of PCR products. Dog blood samples were subjected to the rapid immunochromatographic test (DPP) for detection of anti-Leishmania infantum antibodies. RESULTS: 179 wild mammals were studied and 20.1% had Leishmania DNA successfully detected in at least one sample. Six mammal species were considered infected: Clyomys laticeps, Necromys lasiurus, Nectomys rattus, Rhipidomys macrurus, Didelphis albiventris and Gracilinanus agilis. No significant difference, comparing the proportion of individuals with Leishmania spp., was observed between the sampled areas and wild mammal species. Most of the positive samples were collected from the rodent N. lasiurus, infected by L. amazonensis or L. braziliensis. Moreover, infections by Trypanosoma spp. were detected in N. lasiurus and G. agilis. All 19 dog samples were positive by DPP; however, only three (15.8%) were confirmed by PCR assays. DNA sequences of ITS1 dog amplicons showed 100% identity with L. infantum sequence. CONCLUSIONS: The results suggest the participation of six species of wild mammals in the enzootic transmission of Leishmania spp. in FD. This is the first report of L. amazonensis in N. lasiurus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-0780-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-43860962015-04-07 Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah Cardoso, Rebecca Martins de Araújo, Nadjar Nitz Silva Lociks Romero, Gustavo Adolfo Sierra Souza, Thaís Tâmara Castro Minuzzi Dietrich, Ana Gabriela Mendes, Júnio Donizette Reis, Marcelo Lima Ferreira, Jônatas Barbosa Cavalcante Hecht, Mariana Machado Gurgel-Gonçalves, Rodrigo Parasit Vectors Research BACKGROUND: Wild, synanthropic and domestic mammals act as hosts and/or reservoirs of several Leishmania spp. Studies on possible reservoirs of Leishmania in different areas are fundamental to understand host-parasite interactions and develop strategies for the surveillance and control of leishmaniasis. In the present study, we evaluated the Leishmania spp. occurrence in mammals in two conservation units and their surroundings in Brasília, Federal District (FD), Brazil. METHODS: Small mammals were captured in Brasília National Park (BNP) and Contagem Biological Reserve (CBR) and dogs were sampled in residential areas in their vicinity. Skin and blood samples were evaluated by PCR using different molecular markers (D7 24Sα rRNA and rDNA ITS1). Leishmania species were identified by sequencing of PCR products. Dog blood samples were subjected to the rapid immunochromatographic test (DPP) for detection of anti-Leishmania infantum antibodies. RESULTS: 179 wild mammals were studied and 20.1% had Leishmania DNA successfully detected in at least one sample. Six mammal species were considered infected: Clyomys laticeps, Necromys lasiurus, Nectomys rattus, Rhipidomys macrurus, Didelphis albiventris and Gracilinanus agilis. No significant difference, comparing the proportion of individuals with Leishmania spp., was observed between the sampled areas and wild mammal species. Most of the positive samples were collected from the rodent N. lasiurus, infected by L. amazonensis or L. braziliensis. Moreover, infections by Trypanosoma spp. were detected in N. lasiurus and G. agilis. All 19 dog samples were positive by DPP; however, only three (15.8%) were confirmed by PCR assays. DNA sequences of ITS1 dog amplicons showed 100% identity with L. infantum sequence. CONCLUSIONS: The results suggest the participation of six species of wild mammals in the enzootic transmission of Leishmania spp. in FD. This is the first report of L. amazonensis in N. lasiurus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-0780-y) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-21 /pmc/articles/PMC4386096/ /pubmed/25889365 http://dx.doi.org/10.1186/s13071-015-0780-y Text en © Cardoso et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Cardoso, Rebecca Martins
de Araújo, Nadjar Nitz Silva Lociks
Romero, Gustavo Adolfo Sierra
Souza, Thaís Tâmara Castro Minuzzi
Dietrich, Ana Gabriela
Mendes, Júnio Donizette
Reis, Marcelo Lima
Ferreira, Jônatas Barbosa Cavalcante
Hecht, Mariana Machado
Gurgel-Gonçalves, Rodrigo
Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah
title Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah
title_full Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah
title_fullStr Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah
title_full_unstemmed Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah
title_short Expanding the knowledge about Leishmania species in wild mammals and dogs in the Brazilian savannah
title_sort expanding the knowledge about leishmania species in wild mammals and dogs in the brazilian savannah
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386096/
https://www.ncbi.nlm.nih.gov/pubmed/25889365
http://dx.doi.org/10.1186/s13071-015-0780-y
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