Cargando…

Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides

The effect of pesticides on nicotinamide adenine dinucleotide phosphate hydrogen (NADPH), including its level and relationship with the T helper 1 (Th1)/Th2 ratio, in patients suffering from non-Hodgkin lymphoma (NHL) was investigated. One hundred newly diagnosed patients with aggressive NHL (53 men...

Descripción completa

Detalles Bibliográficos
Autores principales: Zahzeh, Meriem Rabia, Loukidi, Bouchra, Meziane, Warda, Haddouche, Mustapha, Mesli, Naima, Zouaoui, Zahia, Aribi, Mourad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386798/
https://www.ncbi.nlm.nih.gov/pubmed/25878515
http://dx.doi.org/10.2147/JBM.S78759
_version_ 1782365211472166912
author Zahzeh, Meriem Rabia
Loukidi, Bouchra
Meziane, Warda
Haddouche, Mustapha
Mesli, Naima
Zouaoui, Zahia
Aribi, Mourad
author_facet Zahzeh, Meriem Rabia
Loukidi, Bouchra
Meziane, Warda
Haddouche, Mustapha
Mesli, Naima
Zouaoui, Zahia
Aribi, Mourad
author_sort Zahzeh, Meriem Rabia
collection PubMed
description The effect of pesticides on nicotinamide adenine dinucleotide phosphate hydrogen (NADPH), including its level and relationship with the T helper 1 (Th1)/Th2 ratio, in patients suffering from non-Hodgkin lymphoma (NHL) was investigated. One hundred newly diagnosed patients with aggressive NHL (53 men, 47 women) and 40 healthy age-, sex-, and body mass index-matched controls (23 men, 17 women), exposed or not to pesticides, were recruited for a cross-sectional study conducted at the Clinical Hematology Departments of Tlemcen and Sidi Bel-Abbès University Medical Centers in the northwest of Algeria. NADPH levels were significantly increased in patients compared with controls; and in exposed patients compared with those not exposed, and controls (one-way analysis of variance; P=0.000). Albumin, glutathione peroxidase, superoxide dismutase, catalase activity, and oxygen radical absorbance capacity levels were significantly decreased in patients compared with in the control group. Oxygen radical absorbance capacity levels were significantly decreased in exposed patients compared with in unexposed patients; however, malondialdehyde levels were significantly increased in exposed patients when compared with controls and unexposed patients. Protein carbonyl and xanthine oxidase levels were significantly increased in exposed patients compared with controls; meanwhile, there were no significant differences between the two patient groups or between unexposed patients and controls. The Th1/Th2 ratio was significantly decreased in patients when compared with controls; the neutrophil-to-lymphocyte ratio was significantly increased (for both comparisons, P<0.001). In addition, NADPH was strongly associated with NHL (Mantel–Haenszel common odds ratio estimate =5.55; 95% confidence interval, 2.22–13.88; P=0.000). Moreover, NADPH levels were significantly negatively related to the Th1/Th2 ratio, either in exposed patients or in unexposed patients (respectively, r=−0.498 [P=0.004] and r=−0.327 [P=0.006]). In conclusion, pesticide exposure was strongly associated with NADPH alteration in NHL. The relationship between NADPH and Th1/Th2 ratio should focus on new therapeutic strategies for the disease.
format Online
Article
Text
id pubmed-4386798
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-43867982015-04-15 Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides Zahzeh, Meriem Rabia Loukidi, Bouchra Meziane, Warda Haddouche, Mustapha Mesli, Naima Zouaoui, Zahia Aribi, Mourad J Blood Med Original Research The effect of pesticides on nicotinamide adenine dinucleotide phosphate hydrogen (NADPH), including its level and relationship with the T helper 1 (Th1)/Th2 ratio, in patients suffering from non-Hodgkin lymphoma (NHL) was investigated. One hundred newly diagnosed patients with aggressive NHL (53 men, 47 women) and 40 healthy age-, sex-, and body mass index-matched controls (23 men, 17 women), exposed or not to pesticides, were recruited for a cross-sectional study conducted at the Clinical Hematology Departments of Tlemcen and Sidi Bel-Abbès University Medical Centers in the northwest of Algeria. NADPH levels were significantly increased in patients compared with controls; and in exposed patients compared with those not exposed, and controls (one-way analysis of variance; P=0.000). Albumin, glutathione peroxidase, superoxide dismutase, catalase activity, and oxygen radical absorbance capacity levels were significantly decreased in patients compared with in the control group. Oxygen radical absorbance capacity levels were significantly decreased in exposed patients compared with in unexposed patients; however, malondialdehyde levels were significantly increased in exposed patients when compared with controls and unexposed patients. Protein carbonyl and xanthine oxidase levels were significantly increased in exposed patients compared with controls; meanwhile, there were no significant differences between the two patient groups or between unexposed patients and controls. The Th1/Th2 ratio was significantly decreased in patients when compared with controls; the neutrophil-to-lymphocyte ratio was significantly increased (for both comparisons, P<0.001). In addition, NADPH was strongly associated with NHL (Mantel–Haenszel common odds ratio estimate =5.55; 95% confidence interval, 2.22–13.88; P=0.000). Moreover, NADPH levels were significantly negatively related to the Th1/Th2 ratio, either in exposed patients or in unexposed patients (respectively, r=−0.498 [P=0.004] and r=−0.327 [P=0.006]). In conclusion, pesticide exposure was strongly associated with NADPH alteration in NHL. The relationship between NADPH and Th1/Th2 ratio should focus on new therapeutic strategies for the disease. Dove Medical Press 2015-03-31 /pmc/articles/PMC4386798/ /pubmed/25878515 http://dx.doi.org/10.2147/JBM.S78759 Text en © 2015 Zahzeh et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zahzeh, Meriem Rabia
Loukidi, Bouchra
Meziane, Warda
Haddouche, Mustapha
Mesli, Naima
Zouaoui, Zahia
Aribi, Mourad
Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides
title Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides
title_full Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides
title_fullStr Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides
title_full_unstemmed Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides
title_short Relationship between NADPH and Th1/Th2 ratio in patients with non-Hodgkin lymphoma who have been exposed to pesticides
title_sort relationship between nadph and th1/th2 ratio in patients with non-hodgkin lymphoma who have been exposed to pesticides
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386798/
https://www.ncbi.nlm.nih.gov/pubmed/25878515
http://dx.doi.org/10.2147/JBM.S78759
work_keys_str_mv AT zahzehmeriemrabia relationshipbetweennadphandth1th2ratioinpatientswithnonhodgkinlymphomawhohavebeenexposedtopesticides
AT loukidibouchra relationshipbetweennadphandth1th2ratioinpatientswithnonhodgkinlymphomawhohavebeenexposedtopesticides
AT mezianewarda relationshipbetweennadphandth1th2ratioinpatientswithnonhodgkinlymphomawhohavebeenexposedtopesticides
AT haddouchemustapha relationshipbetweennadphandth1th2ratioinpatientswithnonhodgkinlymphomawhohavebeenexposedtopesticides
AT meslinaima relationshipbetweennadphandth1th2ratioinpatientswithnonhodgkinlymphomawhohavebeenexposedtopesticides
AT zouaouizahia relationshipbetweennadphandth1th2ratioinpatientswithnonhodgkinlymphomawhohavebeenexposedtopesticides
AT aribimourad relationshipbetweennadphandth1th2ratioinpatientswithnonhodgkinlymphomawhohavebeenexposedtopesticides