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Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland
Cell–matrix interaction is required for tissue development. Laminin, a major constituent of the basement membrane, is important for structural support and as a ligand in tissue development. Laminin has 19 isoforms, which are determined by combinational assembly of five α, three β, and three γ chains...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387244/ https://www.ncbi.nlm.nih.gov/pubmed/25861129 http://dx.doi.org/10.1267/ahc.14031 |
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author | Ramadhani, Dini Tsukada, Takehiro Fujiwara, Ken Azuma, Morio Kikuchi, Motoshi Yashiro, Takashi |
author_facet | Ramadhani, Dini Tsukada, Takehiro Fujiwara, Ken Azuma, Morio Kikuchi, Motoshi Yashiro, Takashi |
author_sort | Ramadhani, Dini |
collection | PubMed |
description | Cell–matrix interaction is required for tissue development. Laminin, a major constituent of the basement membrane, is important for structural support and as a ligand in tissue development. Laminin has 19 isoforms, which are determined by combinational assembly of five α, three β, and three γ chains (eg, laminin 121 is α1, β2, and γ1). However, no report has identified the laminin isoforms expressed during pituitary development. We used in situ hybridization to investigate all laminin chains expressed during rat anterior pituitary development. The α5 chain was expressed during early pituitary development (embryonic day 12.5–15.5). Expression of α1 and α4 chains was noted in vasculature cells at embryonic day 19.5, but later diminished. The α1 chain was re-expressed in parenchymal cells of anterior lobe from postnatal day 10 (P10), while the α4 chain was present in vasculature cells from P30. The α2 and α3 chains were transiently expressed in vasculature cells and anterior lobe, respectively, only at P30. Widespread distribution of β and γ chains was also observed during development. These findings suggest that numerous laminin isoforms are involved in anterior pituitary gland development and that alteration of the expression pattern is required for proper development of the gland. |
format | Online Article Text |
id | pubmed-4387244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY |
record_format | MEDLINE/PubMed |
spelling | pubmed-43872442015-04-08 Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland Ramadhani, Dini Tsukada, Takehiro Fujiwara, Ken Azuma, Morio Kikuchi, Motoshi Yashiro, Takashi Acta Histochem Cytochem Regular Article Cell–matrix interaction is required for tissue development. Laminin, a major constituent of the basement membrane, is important for structural support and as a ligand in tissue development. Laminin has 19 isoforms, which are determined by combinational assembly of five α, three β, and three γ chains (eg, laminin 121 is α1, β2, and γ1). However, no report has identified the laminin isoforms expressed during pituitary development. We used in situ hybridization to investigate all laminin chains expressed during rat anterior pituitary development. The α5 chain was expressed during early pituitary development (embryonic day 12.5–15.5). Expression of α1 and α4 chains was noted in vasculature cells at embryonic day 19.5, but later diminished. The α1 chain was re-expressed in parenchymal cells of anterior lobe from postnatal day 10 (P10), while the α4 chain was present in vasculature cells from P30. The α2 and α3 chains were transiently expressed in vasculature cells and anterior lobe, respectively, only at P30. Widespread distribution of β and γ chains was also observed during development. These findings suggest that numerous laminin isoforms are involved in anterior pituitary gland development and that alteration of the expression pattern is required for proper development of the gland. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2014-10-31 2014-10-23 /pmc/articles/PMC4387244/ /pubmed/25861129 http://dx.doi.org/10.1267/ahc.14031 Text en 2014 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Ramadhani, Dini Tsukada, Takehiro Fujiwara, Ken Azuma, Morio Kikuchi, Motoshi Yashiro, Takashi Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland |
title | Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland |
title_full | Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland |
title_fullStr | Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland |
title_full_unstemmed | Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland |
title_short | Changes in Laminin Chain Expression in Pre- and Postnatal Rat Pituitary Gland |
title_sort | changes in laminin chain expression in pre- and postnatal rat pituitary gland |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387244/ https://www.ncbi.nlm.nih.gov/pubmed/25861129 http://dx.doi.org/10.1267/ahc.14031 |
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