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Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis
Adhesion capability to fish mucus, which can be affected by environmental conditions, is considered to be a key virulence factor of Vibrio alginolyticus although the molecular mechanism is still unclear. In the present study, V. alginolyticus was treated with stress conditions including Cu(2+) (50 m...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387256/ https://www.ncbi.nlm.nih.gov/pubmed/25726081 http://dx.doi.org/10.1007/s10482-015-0411-9 |
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author | Kong, Wendi Huang, Lixing Su, Yongquan Qin, Yingxue Ma, Ying Xu, Xiaojin Lin, Mao Zheng, Jiang Yan, Qingpi |
author_facet | Kong, Wendi Huang, Lixing Su, Yongquan Qin, Yingxue Ma, Ying Xu, Xiaojin Lin, Mao Zheng, Jiang Yan, Qingpi |
author_sort | Kong, Wendi |
collection | PubMed |
description | Adhesion capability to fish mucus, which can be affected by environmental conditions, is considered to be a key virulence factor of Vibrio alginolyticus although the molecular mechanism is still unclear. In the present study, V. alginolyticus was treated with stress conditions including Cu(2+) (50 mg/L), Pb(2+) (100 mg/L), Hg(2+) (50 mg/L) and low pH (pH 5). We found these stress treatments were capable of reducing the adhesion of V. alginolyticus, while the expression levels of multiple genes were significantly changed according to the results of high throughput sequencing. The expression of randomly selected genes was confirmed by QPCR, which reinforced the reliability of the sequencing data. Ontology assignments and KEGG pathway analysis indicated that stress treatments affect pathways that may be related to adhesion. Our results identified genes which might play a key role in the adhesion process of V. alginolyticus, which could lay a foundation for further functional analysis of these genes in the process of adhesion. As these genes were sensitive to environmental factors, this may explain why the adhesion process can be influenced by environmental factors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-015-0411-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4387256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-43872562015-04-08 Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis Kong, Wendi Huang, Lixing Su, Yongquan Qin, Yingxue Ma, Ying Xu, Xiaojin Lin, Mao Zheng, Jiang Yan, Qingpi Antonie Van Leeuwenhoek Original Paper Adhesion capability to fish mucus, which can be affected by environmental conditions, is considered to be a key virulence factor of Vibrio alginolyticus although the molecular mechanism is still unclear. In the present study, V. alginolyticus was treated with stress conditions including Cu(2+) (50 mg/L), Pb(2+) (100 mg/L), Hg(2+) (50 mg/L) and low pH (pH 5). We found these stress treatments were capable of reducing the adhesion of V. alginolyticus, while the expression levels of multiple genes were significantly changed according to the results of high throughput sequencing. The expression of randomly selected genes was confirmed by QPCR, which reinforced the reliability of the sequencing data. Ontology assignments and KEGG pathway analysis indicated that stress treatments affect pathways that may be related to adhesion. Our results identified genes which might play a key role in the adhesion process of V. alginolyticus, which could lay a foundation for further functional analysis of these genes in the process of adhesion. As these genes were sensitive to environmental factors, this may explain why the adhesion process can be influenced by environmental factors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-015-0411-9) contains supplementary material, which is available to authorized users. Springer International Publishing 2015-03-01 2015 /pmc/articles/PMC4387256/ /pubmed/25726081 http://dx.doi.org/10.1007/s10482-015-0411-9 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Paper Kong, Wendi Huang, Lixing Su, Yongquan Qin, Yingxue Ma, Ying Xu, Xiaojin Lin, Mao Zheng, Jiang Yan, Qingpi Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis |
title | Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis |
title_full | Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis |
title_fullStr | Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis |
title_full_unstemmed | Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis |
title_short | Investigation of possible molecular mechanisms underlying the regulation of adhesion in Vibrio alginolyticus with comparative transcriptome analysis |
title_sort | investigation of possible molecular mechanisms underlying the regulation of adhesion in vibrio alginolyticus with comparative transcriptome analysis |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387256/ https://www.ncbi.nlm.nih.gov/pubmed/25726081 http://dx.doi.org/10.1007/s10482-015-0411-9 |
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