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Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)

Abstract. Cytogenetic data avalaible for the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), one of the most destructive pests of stored cereal grains, are controversial. Earlier studies focused on single populations and emphasized chromosome number and sex determinati...

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Autores principales: da Silva, Alexandra A., Braga, Lucas S., Guedes, Raul Narciso C., Tavares, Mara G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387383/
https://www.ncbi.nlm.nih.gov/pubmed/25893077
http://dx.doi.org/10.3897/CompCytogen.v9i1.4611
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author da Silva, Alexandra A.
Braga, Lucas S.
Guedes, Raul Narciso C.
Tavares, Mara G.
author_facet da Silva, Alexandra A.
Braga, Lucas S.
Guedes, Raul Narciso C.
Tavares, Mara G.
author_sort da Silva, Alexandra A.
collection PubMed
description Abstract. Cytogenetic data avalaible for the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), one of the most destructive pests of stored cereal grains, are controversial. Earlier studies focused on single populations and emphasized chromosome number and sex determination system. In this paper, the karyotypes of four populations of Sitophilus zeamais were characterized by conventional staining, C-banding and sequential staining with the fluorochromes chromomycin-A(3)/4-6-diamidino-2-phenylindole (CMA(3)/DAPI). The analyses of metaphases obtained from the cerebral ganglia of last instar larvae and the testes of adults showed that the species had 2n = 22 chromosomes, with 10 autosomal pairs and a sex chromosome pair (XX in females and Xy(p) in males). Chromosome number, however, ranged from 2n = 22 to 26 due to the presence of 0–4 supernumerary chromosomes in individuals from the populations of Viçosa, Unai and Porto Alegre. With the exception of the Y chromosome, which was dot-like, all other chromosomes of this species were metacentric, including the supernumeraries. The heterochromatin was present in the centromeric regions of all autosomes and in the centromere of the X chromosome. The B chromosomes were partially or totally heterochromatic, and the Y chromosome was euchromatic. The heterochromatic regions were labeled with C-banding and DAPI, which showed that they were rich in AT base pairs.
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spelling pubmed-43873832015-04-17 Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae) da Silva, Alexandra A. Braga, Lucas S. Guedes, Raul Narciso C. Tavares, Mara G. Comp Cytogenet Research Articles Abstract. Cytogenetic data avalaible for the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae), one of the most destructive pests of stored cereal grains, are controversial. Earlier studies focused on single populations and emphasized chromosome number and sex determination system. In this paper, the karyotypes of four populations of Sitophilus zeamais were characterized by conventional staining, C-banding and sequential staining with the fluorochromes chromomycin-A(3)/4-6-diamidino-2-phenylindole (CMA(3)/DAPI). The analyses of metaphases obtained from the cerebral ganglia of last instar larvae and the testes of adults showed that the species had 2n = 22 chromosomes, with 10 autosomal pairs and a sex chromosome pair (XX in females and Xy(p) in males). Chromosome number, however, ranged from 2n = 22 to 26 due to the presence of 0–4 supernumerary chromosomes in individuals from the populations of Viçosa, Unai and Porto Alegre. With the exception of the Y chromosome, which was dot-like, all other chromosomes of this species were metacentric, including the supernumeraries. The heterochromatin was present in the centromeric regions of all autosomes and in the centromere of the X chromosome. The B chromosomes were partially or totally heterochromatic, and the Y chromosome was euchromatic. The heterochromatic regions were labeled with C-banding and DAPI, which showed that they were rich in AT base pairs. Pensoft Publishers 2015-03-31 /pmc/articles/PMC4387383/ /pubmed/25893077 http://dx.doi.org/10.3897/CompCytogen.v9i1.4611 Text en Alexandra A. da Silva, Lucas S. Braga, Raul Narciso C. Guedes, Mara G. Tavares http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Articles
da Silva, Alexandra A.
Braga, Lucas S.
Guedes, Raul Narciso C.
Tavares, Mara G.
Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)
title Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)
title_full Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)
title_fullStr Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)
title_full_unstemmed Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)
title_short Cytogenetic analyses using C-banding and DAPI/CMA3 staining of four populations of the maize weevil Sitophilus zeamais Motschulsky, 1855 (Coleoptera, Curculionidae)
title_sort cytogenetic analyses using c-banding and dapi/cma3 staining of four populations of the maize weevil sitophilus zeamais motschulsky, 1855 (coleoptera, curculionidae)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387383/
https://www.ncbi.nlm.nih.gov/pubmed/25893077
http://dx.doi.org/10.3897/CompCytogen.v9i1.4611
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