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Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms

CXCL10 (IP-10) is a small 10 kDa chemokine with antimicrobial activity. It is induced by IFN-γ, chemoattracts mononuclear cells, and promotes adhesion of T cells. Recently, we detected CXCL10 on the surface of the skin and in the oral cavity. In the current study, we used broth microdilution and rad...

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Autores principales: Holdren, Grant O., Rosenthal, David J., Yang, Jianyi, Bates, Amber M., Fischer, Carol L., Zhang, Yang, Brogden, Nicole K., Brogden, Kim A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387564/
https://www.ncbi.nlm.nih.gov/pubmed/25859394
http://dx.doi.org/10.3390/antibiotics3040527
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author Holdren, Grant O.
Rosenthal, David J.
Yang, Jianyi
Bates, Amber M.
Fischer, Carol L.
Zhang, Yang
Brogden, Nicole K.
Brogden, Kim A.
author_facet Holdren, Grant O.
Rosenthal, David J.
Yang, Jianyi
Bates, Amber M.
Fischer, Carol L.
Zhang, Yang
Brogden, Nicole K.
Brogden, Kim A.
author_sort Holdren, Grant O.
collection PubMed
description CXCL10 (IP-10) is a small 10 kDa chemokine with antimicrobial activity. It is induced by IFN-γ, chemoattracts mononuclear cells, and promotes adhesion of T cells. Recently, we detected CXCL10 on the surface of the skin and in the oral cavity. In the current study, we used broth microdilution and radial diffusion assays to show that CXCL10 inhibits the growth of Escherichia coli, Staphylococcus aureus, Corynebacterium jeikeium, Corynebacterium striatum, and Candida albicans HMV4C, but not Corynebacterium bovis, Streptococcus mutans, Streptococcus mitis, Streptococcus sanguinis, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Poryphromonas gingivalis, or C. albicans ATCC 64124. The reason for the selective antimicrobial activity is not yet known. However, antimicrobial activity of CXCL10 may be related to its composition and structure, as a cationic 98 amino acid residue molecule with 10 lysine residues, 7 arginine residues, a total net charge of +11, and a theoretical pI of 9.93. Modeling studies revealed that CXCL10 contains an α-helix at the N-terminal, three anti-parallel β-strands in the middle, and an α-helix at the C-terminal. Thus, CXCL10, when produced on the surface of the skin or in the oral cavity, likely has antimicrobial activity and may enhance innate antimicrobial and cellular responses to the presence of select commensal or opportunistic microorganisms.
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spelling pubmed-43875642015-04-07 Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms Holdren, Grant O. Rosenthal, David J. Yang, Jianyi Bates, Amber M. Fischer, Carol L. Zhang, Yang Brogden, Nicole K. Brogden, Kim A. Antibiotics (Basel) Article CXCL10 (IP-10) is a small 10 kDa chemokine with antimicrobial activity. It is induced by IFN-γ, chemoattracts mononuclear cells, and promotes adhesion of T cells. Recently, we detected CXCL10 on the surface of the skin and in the oral cavity. In the current study, we used broth microdilution and radial diffusion assays to show that CXCL10 inhibits the growth of Escherichia coli, Staphylococcus aureus, Corynebacterium jeikeium, Corynebacterium striatum, and Candida albicans HMV4C, but not Corynebacterium bovis, Streptococcus mutans, Streptococcus mitis, Streptococcus sanguinis, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Poryphromonas gingivalis, or C. albicans ATCC 64124. The reason for the selective antimicrobial activity is not yet known. However, antimicrobial activity of CXCL10 may be related to its composition and structure, as a cationic 98 amino acid residue molecule with 10 lysine residues, 7 arginine residues, a total net charge of +11, and a theoretical pI of 9.93. Modeling studies revealed that CXCL10 contains an α-helix at the N-terminal, three anti-parallel β-strands in the middle, and an α-helix at the C-terminal. Thus, CXCL10, when produced on the surface of the skin or in the oral cavity, likely has antimicrobial activity and may enhance innate antimicrobial and cellular responses to the presence of select commensal or opportunistic microorganisms. MDPI 2014-10-23 /pmc/articles/PMC4387564/ /pubmed/25859394 http://dx.doi.org/10.3390/antibiotics3040527 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Holdren, Grant O.
Rosenthal, David J.
Yang, Jianyi
Bates, Amber M.
Fischer, Carol L.
Zhang, Yang
Brogden, Nicole K.
Brogden, Kim A.
Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms
title Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms
title_full Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms
title_fullStr Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms
title_full_unstemmed Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms
title_short Antimicrobial Activity of Chemokine CXCL10 for Dermal and Oral Microorganisms
title_sort antimicrobial activity of chemokine cxcl10 for dermal and oral microorganisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4387564/
https://www.ncbi.nlm.nih.gov/pubmed/25859394
http://dx.doi.org/10.3390/antibiotics3040527
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