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Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate

The fusion of two distinct prominences into one continuous structure is common during development and typically requires integration of two epithelia and subsequent removal of that intervening epithelium. Using confocal live imaging, we directly observed the cellular processes underlying tissue fusi...

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Autores principales: Kim, Seungil, Lewis, Ace E., Singh, Vivek, Ma, Xuefei, Adelstein, Robert, Bush, Jeffrey O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388528/
https://www.ncbi.nlm.nih.gov/pubmed/25848986
http://dx.doi.org/10.1371/journal.pbio.1002122
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author Kim, Seungil
Lewis, Ace E.
Singh, Vivek
Ma, Xuefei
Adelstein, Robert
Bush, Jeffrey O.
author_facet Kim, Seungil
Lewis, Ace E.
Singh, Vivek
Ma, Xuefei
Adelstein, Robert
Bush, Jeffrey O.
author_sort Kim, Seungil
collection PubMed
description The fusion of two distinct prominences into one continuous structure is common during development and typically requires integration of two epithelia and subsequent removal of that intervening epithelium. Using confocal live imaging, we directly observed the cellular processes underlying tissue fusion, using the secondary palatal shelves as a model. We find that convergence of a multi-layered epithelium into a single-layer epithelium is an essential early step, driven by cell intercalation, and is concurrent to orthogonal cell displacement and epithelial cell extrusion. Functional studies in mice indicate that this process requires an actomyosin contractility pathway involving Rho kinase (ROCK) and myosin light chain kinase (MLCK), culminating in the activation of non-muscle myosin IIA (NMIIA). Together, these data indicate that actomyosin contractility drives cell intercalation and cell extrusion during palate fusion and suggest a general mechanism for tissue fusion in development.
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spelling pubmed-43885282015-04-21 Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate Kim, Seungil Lewis, Ace E. Singh, Vivek Ma, Xuefei Adelstein, Robert Bush, Jeffrey O. PLoS Biol Research Article The fusion of two distinct prominences into one continuous structure is common during development and typically requires integration of two epithelia and subsequent removal of that intervening epithelium. Using confocal live imaging, we directly observed the cellular processes underlying tissue fusion, using the secondary palatal shelves as a model. We find that convergence of a multi-layered epithelium into a single-layer epithelium is an essential early step, driven by cell intercalation, and is concurrent to orthogonal cell displacement and epithelial cell extrusion. Functional studies in mice indicate that this process requires an actomyosin contractility pathway involving Rho kinase (ROCK) and myosin light chain kinase (MLCK), culminating in the activation of non-muscle myosin IIA (NMIIA). Together, these data indicate that actomyosin contractility drives cell intercalation and cell extrusion during palate fusion and suggest a general mechanism for tissue fusion in development. Public Library of Science 2015-04-07 /pmc/articles/PMC4388528/ /pubmed/25848986 http://dx.doi.org/10.1371/journal.pbio.1002122 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Kim, Seungil
Lewis, Ace E.
Singh, Vivek
Ma, Xuefei
Adelstein, Robert
Bush, Jeffrey O.
Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate
title Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate
title_full Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate
title_fullStr Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate
title_full_unstemmed Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate
title_short Convergence and Extrusion Are Required for Normal Fusion of the Mammalian Secondary Palate
title_sort convergence and extrusion are required for normal fusion of the mammalian secondary palate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388528/
https://www.ncbi.nlm.nih.gov/pubmed/25848986
http://dx.doi.org/10.1371/journal.pbio.1002122
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