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Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study
In this technical report we demonstrate a low-cost online unit allowing movement tracking of flagellated bacteria on a single-cell level during fermentation processes. The system’s ability to distinguish different metabolic states (viability) of bacteria by movement velocity was investigated. A flow...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388530/ https://www.ncbi.nlm.nih.gov/pubmed/25849813 http://dx.doi.org/10.1371/journal.pone.0122531 |
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author | Ziegler, Andreas Schock-Kusch, Daniel Bopp, Dominik Dounia, Sandra Rädle, Matthias Stahl, Ulf |
author_facet | Ziegler, Andreas Schock-Kusch, Daniel Bopp, Dominik Dounia, Sandra Rädle, Matthias Stahl, Ulf |
author_sort | Ziegler, Andreas |
collection | PubMed |
description | In this technical report we demonstrate a low-cost online unit allowing movement tracking of flagellated bacteria on a single-cell level during fermentation processes. The system’s ability to distinguish different metabolic states (viability) of bacteria by movement velocity was investigated. A flow-through cuvette with automatically adjustable layer thickness was developed. The cuvette can be used with most commercially available laboratory microscopes equipped with 40× amplification and a digital camera. In addition, an automated sample preparation unit and a software module was developed measuring size, moved distance, and speed of bacteria. In a proof of principle study the movement velocities of Bacillus amyloliquefaciens FZB42 during three batch fermentation processes were investigated. In this process the bacteria went through different metabolic states, vegetative growth, diauxic shift, vegetative growth after diauxic shift, and sporulation. It was shown that the movement velocities during the different metabolic states significantly differ from each other. Therefore, the described setup has the potential to be used as a bacteria viability monitoring tool. In contrast to some other techniques, such as electro-optical techniques, this method can even be used in turbid production media. |
format | Online Article Text |
id | pubmed-4388530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43885302015-04-21 Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study Ziegler, Andreas Schock-Kusch, Daniel Bopp, Dominik Dounia, Sandra Rädle, Matthias Stahl, Ulf PLoS One Research Article In this technical report we demonstrate a low-cost online unit allowing movement tracking of flagellated bacteria on a single-cell level during fermentation processes. The system’s ability to distinguish different metabolic states (viability) of bacteria by movement velocity was investigated. A flow-through cuvette with automatically adjustable layer thickness was developed. The cuvette can be used with most commercially available laboratory microscopes equipped with 40× amplification and a digital camera. In addition, an automated sample preparation unit and a software module was developed measuring size, moved distance, and speed of bacteria. In a proof of principle study the movement velocities of Bacillus amyloliquefaciens FZB42 during three batch fermentation processes were investigated. In this process the bacteria went through different metabolic states, vegetative growth, diauxic shift, vegetative growth after diauxic shift, and sporulation. It was shown that the movement velocities during the different metabolic states significantly differ from each other. Therefore, the described setup has the potential to be used as a bacteria viability monitoring tool. In contrast to some other techniques, such as electro-optical techniques, this method can even be used in turbid production media. Public Library of Science 2015-04-07 /pmc/articles/PMC4388530/ /pubmed/25849813 http://dx.doi.org/10.1371/journal.pone.0122531 Text en © 2015 Ziegler et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ziegler, Andreas Schock-Kusch, Daniel Bopp, Dominik Dounia, Sandra Rädle, Matthias Stahl, Ulf Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study |
title | Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study |
title_full | Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study |
title_fullStr | Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study |
title_full_unstemmed | Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study |
title_short | Single Bacteria Movement Tracking by Online Microscopy – A Proof of Concept Study |
title_sort | single bacteria movement tracking by online microscopy – a proof of concept study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388530/ https://www.ncbi.nlm.nih.gov/pubmed/25849813 http://dx.doi.org/10.1371/journal.pone.0122531 |
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