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Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors

BACKGROUND: The present study was designed to determine whether the Thinprep plus Papanicolaou stain (Thinprep) method is more sensitive than the Cytospin-coupled Wright-Giemsa (WG) stain (Cytospin) method in diagnosis of leptomeningeal metastasis (LM) from malignant solid tumors in cerebrospinal fl...

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Autores principales: Pan, Zhenyu, Yang, Guozi, Wang, Yongxiang, He, Hua, Pang, Xiaochuan, Gao, Yan, Shi, Weiyan, Li, Yu, Dong, Lihua, Song, Yuanyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388538/
https://www.ncbi.nlm.nih.gov/pubmed/25850010
http://dx.doi.org/10.1371/journal.pone.0122016
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author Pan, Zhenyu
Yang, Guozi
Wang, Yongxiang
He, Hua
Pang, Xiaochuan
Gao, Yan
Shi, Weiyan
Li, Yu
Dong, Lihua
Song, Yuanyuan
author_facet Pan, Zhenyu
Yang, Guozi
Wang, Yongxiang
He, Hua
Pang, Xiaochuan
Gao, Yan
Shi, Weiyan
Li, Yu
Dong, Lihua
Song, Yuanyuan
author_sort Pan, Zhenyu
collection PubMed
description BACKGROUND: The present study was designed to determine whether the Thinprep plus Papanicolaou stain (Thinprep) method is more sensitive than the Cytospin-coupled Wright-Giemsa (WG) stain (Cytospin) method in diagnosis of leptomeningeal metastasis (LM) from malignant solid tumors in cerebrospinal fluid (CSF). We also explored if the Thinprep method could be used in the differential diagnosis of the type of primary tumor cells based on the morphology of tumor cells in CSF samples. METHODS: The morphological features of tumor cells in fresh CSF samples were analyzed using both methods. The tumor cell detection rates were compared between the two methods. RESULTS: Using the Thinprep method, we found that each type of tumor cells in the CSF samples had specific identifiable morphological features linked to their primary cancer origins, such as adenocarcinomas originated from the lungs, breast, and stomach, and lung squamous cell carcinomas, small cell lung cancer, large-cell neuroendocrine lung cancer, hepatocellular carcinoma, and malignant melanoma. In a retrospective study with 88 LM patients, cancer cells were detected in 80 out of the 88 CSF samples. In the comparative study with 45 LM patients, the initial detection rate of the Thinprep method was significantly higher than that of the Cytospin method (73.3% vs. 57.8%, P<0.01). The cell morphology was better preserved and subcellular structures were clearer using the Thinprep method, compared to the Cytospin method. CONCLUSIONS: The Thinprep method is more sensitive and suitable for LM diagnosis in CSF in patients with malignant solid tumors than the Cytospin method. The Thinprep method may facilitate primary tumor detection and help design early treatment regimens for LM patients with tumors of unknown primary origin.
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spelling pubmed-43885382015-04-21 Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors Pan, Zhenyu Yang, Guozi Wang, Yongxiang He, Hua Pang, Xiaochuan Gao, Yan Shi, Weiyan Li, Yu Dong, Lihua Song, Yuanyuan PLoS One Research Article BACKGROUND: The present study was designed to determine whether the Thinprep plus Papanicolaou stain (Thinprep) method is more sensitive than the Cytospin-coupled Wright-Giemsa (WG) stain (Cytospin) method in diagnosis of leptomeningeal metastasis (LM) from malignant solid tumors in cerebrospinal fluid (CSF). We also explored if the Thinprep method could be used in the differential diagnosis of the type of primary tumor cells based on the morphology of tumor cells in CSF samples. METHODS: The morphological features of tumor cells in fresh CSF samples were analyzed using both methods. The tumor cell detection rates were compared between the two methods. RESULTS: Using the Thinprep method, we found that each type of tumor cells in the CSF samples had specific identifiable morphological features linked to their primary cancer origins, such as adenocarcinomas originated from the lungs, breast, and stomach, and lung squamous cell carcinomas, small cell lung cancer, large-cell neuroendocrine lung cancer, hepatocellular carcinoma, and malignant melanoma. In a retrospective study with 88 LM patients, cancer cells were detected in 80 out of the 88 CSF samples. In the comparative study with 45 LM patients, the initial detection rate of the Thinprep method was significantly higher than that of the Cytospin method (73.3% vs. 57.8%, P<0.01). The cell morphology was better preserved and subcellular structures were clearer using the Thinprep method, compared to the Cytospin method. CONCLUSIONS: The Thinprep method is more sensitive and suitable for LM diagnosis in CSF in patients with malignant solid tumors than the Cytospin method. The Thinprep method may facilitate primary tumor detection and help design early treatment regimens for LM patients with tumors of unknown primary origin. Public Library of Science 2015-04-07 /pmc/articles/PMC4388538/ /pubmed/25850010 http://dx.doi.org/10.1371/journal.pone.0122016 Text en © 2015 Pan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pan, Zhenyu
Yang, Guozi
Wang, Yongxiang
He, Hua
Pang, Xiaochuan
Gao, Yan
Shi, Weiyan
Li, Yu
Dong, Lihua
Song, Yuanyuan
Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors
title Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors
title_full Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors
title_fullStr Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors
title_full_unstemmed Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors
title_short Thinprep plus Papanicolaou Stain Method Is More Sensitive than Cytospin-Coupled Wright Giems Stain Method in Cerebrospinal Fluid Cytology for Diagnosis of Leptomeningeal Metastasis from Solid Tumors
title_sort thinprep plus papanicolaou stain method is more sensitive than cytospin-coupled wright giems stain method in cerebrospinal fluid cytology for diagnosis of leptomeningeal metastasis from solid tumors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388538/
https://www.ncbi.nlm.nih.gov/pubmed/25850010
http://dx.doi.org/10.1371/journal.pone.0122016
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