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Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments

During limb development, the interdigital mesenchyme has been proposed to play a signaling role instructing morphogenesis of different digit types, as well as undergoing programmed cell death necessary to free digits in animals not adapted for swimming or flying. We have generated a conditional, tam...

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Detalles Bibliográficos
Autores principales: Huang, Bau-Lin, Mackem, Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388723/
https://www.ncbi.nlm.nih.gov/pubmed/25850076
http://dx.doi.org/10.1371/journal.pone.0123325
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author Huang, Bau-Lin
Mackem, Susan
author_facet Huang, Bau-Lin
Mackem, Susan
author_sort Huang, Bau-Lin
collection PubMed
description During limb development, the interdigital mesenchyme has been proposed to play a signaling role instructing morphogenesis of different digit types, as well as undergoing programmed cell death necessary to free digits in animals not adapted for swimming or flying. We have generated a conditional, tamoxifen-dependent Cre line, Bmp2CreER, which drives highly selective recombination restricted to the distal limb mesoderm, largely restricted to the interdigits, and selectively active in digit ligament but not tendon progenitors at later stages. The Bmp2CreER provides a valuable new tool to dissect roles of interdigital mesenchyme and potentially investigate divergence of ligament and tendon lineages.
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spelling pubmed-43887232015-04-21 Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments Huang, Bau-Lin Mackem, Susan PLoS One Research Article During limb development, the interdigital mesenchyme has been proposed to play a signaling role instructing morphogenesis of different digit types, as well as undergoing programmed cell death necessary to free digits in animals not adapted for swimming or flying. We have generated a conditional, tamoxifen-dependent Cre line, Bmp2CreER, which drives highly selective recombination restricted to the distal limb mesoderm, largely restricted to the interdigits, and selectively active in digit ligament but not tendon progenitors at later stages. The Bmp2CreER provides a valuable new tool to dissect roles of interdigital mesenchyme and potentially investigate divergence of ligament and tendon lineages. Public Library of Science 2015-04-07 /pmc/articles/PMC4388723/ /pubmed/25850076 http://dx.doi.org/10.1371/journal.pone.0123325 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Huang, Bau-Lin
Mackem, Susan
Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments
title Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments
title_full Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments
title_fullStr Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments
title_full_unstemmed Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments
title_short Tamoxifen-Dependent, Inducible Bmp2CreER Drives Selective Recombinase Activity in Early Interdigital Mesenchyme and Digit Collateral Ligaments
title_sort tamoxifen-dependent, inducible bmp2creer drives selective recombinase activity in early interdigital mesenchyme and digit collateral ligaments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388723/
https://www.ncbi.nlm.nih.gov/pubmed/25850076
http://dx.doi.org/10.1371/journal.pone.0123325
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