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Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions

Stem cells have remarkable self-renewal ability and differentiation potency, which are critical for tissue repair and tissue homeostasis. Recently it has been found, in many systems (e.g. gut, neurons, and hematopoietic stem cells), that the self-renewal and differentiation balance is maintained whe...

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Detalles Bibliográficos
Autores principales: Bang, Chi, Cheng, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388834/
https://www.ncbi.nlm.nih.gov/pubmed/25849996
http://dx.doi.org/10.1371/journal.pone.0123294
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author Bang, Chi
Cheng, Jun
author_facet Bang, Chi
Cheng, Jun
author_sort Bang, Chi
collection PubMed
description Stem cells have remarkable self-renewal ability and differentiation potency, which are critical for tissue repair and tissue homeostasis. Recently it has been found, in many systems (e.g. gut, neurons, and hematopoietic stem cells), that the self-renewal and differentiation balance is maintained when the stem cells divide asymmetrically. Drosophila male germline stem cells (GSCs), one of the best characterized model systems with well-defined stem cell niches, were reported to divide asymmetrically, where centrosome plays an important role. Utilizing time-lapse live cell imaging, customized tracking, and image processing programs, we found that most acentrosomal GSCs have the spectrosomes reposition from the basal end (wild type) to the apical end close to hub-GSC interface (acentrosomal GSCs). In addition, these apically positioned spectrosomes were mostly stationary while the basally positioned spectrosomes were mobile. For acentrosomal GSCs, their mitotic spindles were still highly oriented and divided asymmetrically with longer mitosis duration, resulting in asymmetric divisions. Moreover, when the spectrosome was knocked out, the centrosomes velocity decreased and centrosomes located closer to hub-GSC interface. We propose that in male GSCs, the spectrosome recruited to the apical end plays a complimentary role in ensuring proper spindle orientation when centrosome function is compromised.
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spelling pubmed-43888342015-04-21 Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions Bang, Chi Cheng, Jun PLoS One Research Article Stem cells have remarkable self-renewal ability and differentiation potency, which are critical for tissue repair and tissue homeostasis. Recently it has been found, in many systems (e.g. gut, neurons, and hematopoietic stem cells), that the self-renewal and differentiation balance is maintained when the stem cells divide asymmetrically. Drosophila male germline stem cells (GSCs), one of the best characterized model systems with well-defined stem cell niches, were reported to divide asymmetrically, where centrosome plays an important role. Utilizing time-lapse live cell imaging, customized tracking, and image processing programs, we found that most acentrosomal GSCs have the spectrosomes reposition from the basal end (wild type) to the apical end close to hub-GSC interface (acentrosomal GSCs). In addition, these apically positioned spectrosomes were mostly stationary while the basally positioned spectrosomes were mobile. For acentrosomal GSCs, their mitotic spindles were still highly oriented and divided asymmetrically with longer mitosis duration, resulting in asymmetric divisions. Moreover, when the spectrosome was knocked out, the centrosomes velocity decreased and centrosomes located closer to hub-GSC interface. We propose that in male GSCs, the spectrosome recruited to the apical end plays a complimentary role in ensuring proper spindle orientation when centrosome function is compromised. Public Library of Science 2015-04-07 /pmc/articles/PMC4388834/ /pubmed/25849996 http://dx.doi.org/10.1371/journal.pone.0123294 Text en © 2015 Bang, Cheng http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bang, Chi
Cheng, Jun
Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions
title Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions
title_full Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions
title_fullStr Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions
title_full_unstemmed Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions
title_short Dynamic Interplay of Spectrosome and Centrosome Organelles in Asymmetric Stem Cell Divisions
title_sort dynamic interplay of spectrosome and centrosome organelles in asymmetric stem cell divisions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388834/
https://www.ncbi.nlm.nih.gov/pubmed/25849996
http://dx.doi.org/10.1371/journal.pone.0123294
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