Cargando…
Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos
Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos of zebrafish is unknown. Therefore, human ADSCs were firstly isolated, and then transd...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388839/ https://www.ncbi.nlm.nih.gov/pubmed/25849455 http://dx.doi.org/10.1371/journal.pone.0123264 |
_version_ | 1782365444399693824 |
---|---|
author | Li, Jin Zeng, Guofang Qi, Yawei Tang, Xudong Zhang, Jingjing Wu, Zeyong Liang, Jie Shi, Lei Liu, Hongwei Zhang, Peihua |
author_facet | Li, Jin Zeng, Guofang Qi, Yawei Tang, Xudong Zhang, Jingjing Wu, Zeyong Liang, Jie Shi, Lei Liu, Hongwei Zhang, Peihua |
author_sort | Li, Jin |
collection | PubMed |
description | Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos of zebrafish is unknown. Therefore, human ADSCs were firstly isolated, and then transduced with lentiviral vector system carrying a green fluorescent protein (GFP) reporter gene, and followed by detection of their cell viability and the expression of cell surface antigens. These GFP-expressing human ADSCs were transplanted into the zebrafish embryos at 3.3–4.3 hour post-fertilization (hpf). Green fluorescent signal, the proliferation and differentiation of human ADSCs in recipient embryos were respectively examined using fluorescent microscopy and immunohistochemical staining. The results indicated that human ADSCs did not change their cell viability and the expression levels of cell surface antigens after GFP transduction. Microscopic examination demonstrated that green fluorescent signals of GFP expressed in the transplanted cells were observed in the embryos and larva fish at post-transplantation. The positive staining of Ki-67 revealed the survival and proliferation of human ADSCs in fish larvae after transplantation. The expression of CD105 was observable in the xenotransplanted ADSCs, but CD31 expression was undetectable. Therefore, our results indicate that human ADSCs xenotransplanted in the zebrafish embryos not only can survive and proliferate at across-species circumstance, but also seem to maintain their undifferentiation status in a short term. This xenograft model of zebrafish embryos may provide a promising and useful technical platform for the investigation of biology and physiology of stem cells in vivo. |
format | Online Article Text |
id | pubmed-4388839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43888392015-04-21 Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos Li, Jin Zeng, Guofang Qi, Yawei Tang, Xudong Zhang, Jingjing Wu, Zeyong Liang, Jie Shi, Lei Liu, Hongwei Zhang, Peihua PLoS One Research Article Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos of zebrafish is unknown. Therefore, human ADSCs were firstly isolated, and then transduced with lentiviral vector system carrying a green fluorescent protein (GFP) reporter gene, and followed by detection of their cell viability and the expression of cell surface antigens. These GFP-expressing human ADSCs were transplanted into the zebrafish embryos at 3.3–4.3 hour post-fertilization (hpf). Green fluorescent signal, the proliferation and differentiation of human ADSCs in recipient embryos were respectively examined using fluorescent microscopy and immunohistochemical staining. The results indicated that human ADSCs did not change their cell viability and the expression levels of cell surface antigens after GFP transduction. Microscopic examination demonstrated that green fluorescent signals of GFP expressed in the transplanted cells were observed in the embryos and larva fish at post-transplantation. The positive staining of Ki-67 revealed the survival and proliferation of human ADSCs in fish larvae after transplantation. The expression of CD105 was observable in the xenotransplanted ADSCs, but CD31 expression was undetectable. Therefore, our results indicate that human ADSCs xenotransplanted in the zebrafish embryos not only can survive and proliferate at across-species circumstance, but also seem to maintain their undifferentiation status in a short term. This xenograft model of zebrafish embryos may provide a promising and useful technical platform for the investigation of biology and physiology of stem cells in vivo. Public Library of Science 2015-04-07 /pmc/articles/PMC4388839/ /pubmed/25849455 http://dx.doi.org/10.1371/journal.pone.0123264 Text en © 2015 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Jin Zeng, Guofang Qi, Yawei Tang, Xudong Zhang, Jingjing Wu, Zeyong Liang, Jie Shi, Lei Liu, Hongwei Zhang, Peihua Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos |
title | Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos |
title_full | Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos |
title_fullStr | Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos |
title_full_unstemmed | Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos |
title_short | Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos |
title_sort | xenotransplantation of human adipose-derived stem cells in zebrafish embryos |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388839/ https://www.ncbi.nlm.nih.gov/pubmed/25849455 http://dx.doi.org/10.1371/journal.pone.0123264 |
work_keys_str_mv | AT lijin xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT zengguofang xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT qiyawei xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT tangxudong xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT zhangjingjing xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT wuzeyong xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT liangjie xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT shilei xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT liuhongwei xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos AT zhangpeihua xenotransplantationofhumanadiposederivedstemcellsinzebrafishembryos |