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Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos

Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos of zebrafish is unknown. Therefore, human ADSCs were firstly isolated, and then transd...

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Autores principales: Li, Jin, Zeng, Guofang, Qi, Yawei, Tang, Xudong, Zhang, Jingjing, Wu, Zeyong, Liang, Jie, Shi, Lei, Liu, Hongwei, Zhang, Peihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388839/
https://www.ncbi.nlm.nih.gov/pubmed/25849455
http://dx.doi.org/10.1371/journal.pone.0123264
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author Li, Jin
Zeng, Guofang
Qi, Yawei
Tang, Xudong
Zhang, Jingjing
Wu, Zeyong
Liang, Jie
Shi, Lei
Liu, Hongwei
Zhang, Peihua
author_facet Li, Jin
Zeng, Guofang
Qi, Yawei
Tang, Xudong
Zhang, Jingjing
Wu, Zeyong
Liang, Jie
Shi, Lei
Liu, Hongwei
Zhang, Peihua
author_sort Li, Jin
collection PubMed
description Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos of zebrafish is unknown. Therefore, human ADSCs were firstly isolated, and then transduced with lentiviral vector system carrying a green fluorescent protein (GFP) reporter gene, and followed by detection of their cell viability and the expression of cell surface antigens. These GFP-expressing human ADSCs were transplanted into the zebrafish embryos at 3.3–4.3 hour post-fertilization (hpf). Green fluorescent signal, the proliferation and differentiation of human ADSCs in recipient embryos were respectively examined using fluorescent microscopy and immunohistochemical staining. The results indicated that human ADSCs did not change their cell viability and the expression levels of cell surface antigens after GFP transduction. Microscopic examination demonstrated that green fluorescent signals of GFP expressed in the transplanted cells were observed in the embryos and larva fish at post-transplantation. The positive staining of Ki-67 revealed the survival and proliferation of human ADSCs in fish larvae after transplantation. The expression of CD105 was observable in the xenotransplanted ADSCs, but CD31 expression was undetectable. Therefore, our results indicate that human ADSCs xenotransplanted in the zebrafish embryos not only can survive and proliferate at across-species circumstance, but also seem to maintain their undifferentiation status in a short term. This xenograft model of zebrafish embryos may provide a promising and useful technical platform for the investigation of biology and physiology of stem cells in vivo.
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spelling pubmed-43888392015-04-21 Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos Li, Jin Zeng, Guofang Qi, Yawei Tang, Xudong Zhang, Jingjing Wu, Zeyong Liang, Jie Shi, Lei Liu, Hongwei Zhang, Peihua PLoS One Research Article Zebrafish is a widely used animal model with well-characterized background in developmental biology. The fate of human adipose-derived stem cells (ADSCs) after their xenotransplantation into the developing embryos of zebrafish is unknown. Therefore, human ADSCs were firstly isolated, and then transduced with lentiviral vector system carrying a green fluorescent protein (GFP) reporter gene, and followed by detection of their cell viability and the expression of cell surface antigens. These GFP-expressing human ADSCs were transplanted into the zebrafish embryos at 3.3–4.3 hour post-fertilization (hpf). Green fluorescent signal, the proliferation and differentiation of human ADSCs in recipient embryos were respectively examined using fluorescent microscopy and immunohistochemical staining. The results indicated that human ADSCs did not change their cell viability and the expression levels of cell surface antigens after GFP transduction. Microscopic examination demonstrated that green fluorescent signals of GFP expressed in the transplanted cells were observed in the embryos and larva fish at post-transplantation. The positive staining of Ki-67 revealed the survival and proliferation of human ADSCs in fish larvae after transplantation. The expression of CD105 was observable in the xenotransplanted ADSCs, but CD31 expression was undetectable. Therefore, our results indicate that human ADSCs xenotransplanted in the zebrafish embryos not only can survive and proliferate at across-species circumstance, but also seem to maintain their undifferentiation status in a short term. This xenograft model of zebrafish embryos may provide a promising and useful technical platform for the investigation of biology and physiology of stem cells in vivo. Public Library of Science 2015-04-07 /pmc/articles/PMC4388839/ /pubmed/25849455 http://dx.doi.org/10.1371/journal.pone.0123264 Text en © 2015 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Jin
Zeng, Guofang
Qi, Yawei
Tang, Xudong
Zhang, Jingjing
Wu, Zeyong
Liang, Jie
Shi, Lei
Liu, Hongwei
Zhang, Peihua
Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos
title Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos
title_full Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos
title_fullStr Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos
title_full_unstemmed Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos
title_short Xenotransplantation of Human Adipose-Derived Stem Cells in Zebrafish Embryos
title_sort xenotransplantation of human adipose-derived stem cells in zebrafish embryos
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388839/
https://www.ncbi.nlm.nih.gov/pubmed/25849455
http://dx.doi.org/10.1371/journal.pone.0123264
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