Cargando…

Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis

The detection of antibodies is useful to diagnose and/or to classify autoimmune diseases as connective tissue diseases and vasculitis. Zenit RA is a fully automated immunoanalyzer. The aim of this study was to compare the predictive and discriminative performance of the Zenit RA anti-cyclic citrulli...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghillani, P., Dufat, L., Himeur, S., Miyara, M., Amoura, Z., Musset, L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389018/
https://www.ncbi.nlm.nih.gov/pubmed/26000125
http://dx.doi.org/10.1007/s13317-012-0032-5
_version_ 1782365469536157696
author Ghillani, P.
Dufat, L.
Himeur, S.
Miyara, M.
Amoura, Z.
Musset, L.
author_facet Ghillani, P.
Dufat, L.
Himeur, S.
Miyara, M.
Amoura, Z.
Musset, L.
author_sort Ghillani, P.
collection PubMed
description The detection of antibodies is useful to diagnose and/or to classify autoimmune diseases as connective tissue diseases and vasculitis. Zenit RA is a fully automated immunoanalyzer. The aim of this study was to compare the predictive and discriminative performance of the Zenit RA anti-cyclic citrullinated peptide (CCP), anti-cardiolipin (aCL) and anti-β 2 glycoprotein 1 (aB2GP1) tests to conventional ELISAs on clinically well-defined groups of patients and to daily evaluate the determination of anti-extractable nuclear antigen (ENA), anti-double stranded DNA (dsDNA), anti-myeloperoxidase (MPO) and anti-proteinase 3 (PR3) antibodies in a hospital laboratory. Reagents available on Zenit RA analyzer exhibit good diagnostic performances, regarding sensitivity, specificity, positive and negative predictive values. Global agreements between Zenit RA and conventional tests were from 90 to 98 % (Kappa-values ranging 0.56–0.94): 96 % for anti-CCP, 90–94 % for aCL and aB2GP1, 94 % for anti-dsDNA, 97 % for anti-ENA, 98 % for anti-MPO and 95 % for anti-PR3 antibodies. Zenit RA analyzer is easy to rapidly detect the most common autoantibodies in autoimmune diseases. This system has a potential to provide clinically useful data within a short time. Because of the flexibility of its work modalities, it is well adapted to determine antigenic specificities in daily practice.
format Online
Article
Text
id pubmed-4389018
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Springer International Publishing
record_format MEDLINE/PubMed
spelling pubmed-43890182015-05-21 Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis Ghillani, P. Dufat, L. Himeur, S. Miyara, M. Amoura, Z. Musset, L. Auto Immun Highlights Original Article The detection of antibodies is useful to diagnose and/or to classify autoimmune diseases as connective tissue diseases and vasculitis. Zenit RA is a fully automated immunoanalyzer. The aim of this study was to compare the predictive and discriminative performance of the Zenit RA anti-cyclic citrullinated peptide (CCP), anti-cardiolipin (aCL) and anti-β 2 glycoprotein 1 (aB2GP1) tests to conventional ELISAs on clinically well-defined groups of patients and to daily evaluate the determination of anti-extractable nuclear antigen (ENA), anti-double stranded DNA (dsDNA), anti-myeloperoxidase (MPO) and anti-proteinase 3 (PR3) antibodies in a hospital laboratory. Reagents available on Zenit RA analyzer exhibit good diagnostic performances, regarding sensitivity, specificity, positive and negative predictive values. Global agreements between Zenit RA and conventional tests were from 90 to 98 % (Kappa-values ranging 0.56–0.94): 96 % for anti-CCP, 90–94 % for aCL and aB2GP1, 94 % for anti-dsDNA, 97 % for anti-ENA, 98 % for anti-MPO and 95 % for anti-PR3 antibodies. Zenit RA analyzer is easy to rapidly detect the most common autoantibodies in autoimmune diseases. This system has a potential to provide clinically useful data within a short time. Because of the flexibility of its work modalities, it is well adapted to determine antigenic specificities in daily practice. Springer International Publishing 2012-03-21 /pmc/articles/PMC4389018/ /pubmed/26000125 http://dx.doi.org/10.1007/s13317-012-0032-5 Text en © Springer-Verlag 2012
spellingShingle Original Article
Ghillani, P.
Dufat, L.
Himeur, S.
Miyara, M.
Amoura, Z.
Musset, L.
Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis
title Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis
title_full Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis
title_fullStr Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis
title_full_unstemmed Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis
title_short Routine use of Zenit RA, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis
title_sort routine use of zenit ra, a novel chemiluminescent immunoanalyzer in autoimmune disease diagnosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389018/
https://www.ncbi.nlm.nih.gov/pubmed/26000125
http://dx.doi.org/10.1007/s13317-012-0032-5
work_keys_str_mv AT ghillanip routineuseofzenitraanovelchemiluminescentimmunoanalyzerinautoimmunediseasediagnosis
AT dufatl routineuseofzenitraanovelchemiluminescentimmunoanalyzerinautoimmunediseasediagnosis
AT himeurs routineuseofzenitraanovelchemiluminescentimmunoanalyzerinautoimmunediseasediagnosis
AT miyaram routineuseofzenitraanovelchemiluminescentimmunoanalyzerinautoimmunediseasediagnosis
AT amouraz routineuseofzenitraanovelchemiluminescentimmunoanalyzerinautoimmunediseasediagnosis
AT mussetl routineuseofzenitraanovelchemiluminescentimmunoanalyzerinautoimmunediseasediagnosis