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The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats

OBJECTIVE: To explore the immunological mechanisms underlying the effect of chronic intermittent hypobaric hypoxia (CIHH) pretreatment on collagen-induced arthritis (CIA) in rat. METHODS: Fifty-four adult male Sprague–Dawley rats were used in the experiment. Arthritis in CIA rats (n=18) was induced...

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Autores principales: Shi, Min, Cui, Fang, Liu, Ai-Jing, Ma, Hui-Jie, Cheng, Ming, Song, Shu-Xia, Yuan, Fang, Li, De-Pei, Zhang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389442/
https://www.ncbi.nlm.nih.gov/pubmed/25861246
http://dx.doi.org/10.1186/s12950-015-0068-1
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author Shi, Min
Cui, Fang
Liu, Ai-Jing
Ma, Hui-Jie
Cheng, Ming
Song, Shu-Xia
Yuan, Fang
Li, De-Pei
Zhang, Yi
author_facet Shi, Min
Cui, Fang
Liu, Ai-Jing
Ma, Hui-Jie
Cheng, Ming
Song, Shu-Xia
Yuan, Fang
Li, De-Pei
Zhang, Yi
author_sort Shi, Min
collection PubMed
description OBJECTIVE: To explore the immunological mechanisms underlying the effect of chronic intermittent hypobaric hypoxia (CIHH) pretreatment on collagen-induced arthritis (CIA) in rat. METHODS: Fifty-four adult male Sprague–Dawley rats were used in the experiment. Arthritis in CIA rats (n=18) was induced by injection of collagen. The CIHH+CIA rats (n=18) were treated with CIHH (simulated 3000 m altitude, 5 hours per day for 28 days, PO(2)=108.8 mmHg) before CIA. The control rats (n=18) were not given any treatment. RESULTS: (1) Incidence rate of CIA in CIHH+CIA rats was significantly lower than that in CIA rats (P<0.05). (2) The paw thickness and arthritis index (AI) value in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (3) The hyperplasia with inflammatory infiltration in synovial tissue of joints in CIHH+CIA rats was much alleviative compared with CIA rats. (4) TNF-α, IFN-γ, IL-4 and IL-17 in synovial tissue of joint and serum in CIHH+CIA rats were decreased compared with CIA rats (P<0.05). (5) The number of CD4-positive T-lymphocytes and the ratio of CD4/CD8 T-lymphocytes in peripheral blood in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (6) The protein expression of HIF-1α and NF-κB in synovial tissue of joint in CIHH+CIA rats was decreased compared with CIA rats (P<0.05). CONCLUSION: CIHH pretreatment has a protective effect against collagen-induced arthritis in rat through down-regulation of HIF-1α and NF-κB, inhibition of inflammatory cytokines TNF-α and IL-17, and balance in CD4/CD8 and Th1/Th2 T lymphocytes.
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spelling pubmed-43894422015-04-09 The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats Shi, Min Cui, Fang Liu, Ai-Jing Ma, Hui-Jie Cheng, Ming Song, Shu-Xia Yuan, Fang Li, De-Pei Zhang, Yi J Inflamm (Lond) Research OBJECTIVE: To explore the immunological mechanisms underlying the effect of chronic intermittent hypobaric hypoxia (CIHH) pretreatment on collagen-induced arthritis (CIA) in rat. METHODS: Fifty-four adult male Sprague–Dawley rats were used in the experiment. Arthritis in CIA rats (n=18) was induced by injection of collagen. The CIHH+CIA rats (n=18) were treated with CIHH (simulated 3000 m altitude, 5 hours per day for 28 days, PO(2)=108.8 mmHg) before CIA. The control rats (n=18) were not given any treatment. RESULTS: (1) Incidence rate of CIA in CIHH+CIA rats was significantly lower than that in CIA rats (P<0.05). (2) The paw thickness and arthritis index (AI) value in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (3) The hyperplasia with inflammatory infiltration in synovial tissue of joints in CIHH+CIA rats was much alleviative compared with CIA rats. (4) TNF-α, IFN-γ, IL-4 and IL-17 in synovial tissue of joint and serum in CIHH+CIA rats were decreased compared with CIA rats (P<0.05). (5) The number of CD4-positive T-lymphocytes and the ratio of CD4/CD8 T-lymphocytes in peripheral blood in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (6) The protein expression of HIF-1α and NF-κB in synovial tissue of joint in CIHH+CIA rats was decreased compared with CIA rats (P<0.05). CONCLUSION: CIHH pretreatment has a protective effect against collagen-induced arthritis in rat through down-regulation of HIF-1α and NF-κB, inhibition of inflammatory cytokines TNF-α and IL-17, and balance in CD4/CD8 and Th1/Th2 T lymphocytes. BioMed Central 2015-03-25 /pmc/articles/PMC4389442/ /pubmed/25861246 http://dx.doi.org/10.1186/s12950-015-0068-1 Text en © Shi et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Shi, Min
Cui, Fang
Liu, Ai-Jing
Ma, Hui-Jie
Cheng, Ming
Song, Shu-Xia
Yuan, Fang
Li, De-Pei
Zhang, Yi
The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
title The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
title_full The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
title_fullStr The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
title_full_unstemmed The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
title_short The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
title_sort protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389442/
https://www.ncbi.nlm.nih.gov/pubmed/25861246
http://dx.doi.org/10.1186/s12950-015-0068-1
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