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Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus
Functional metagenomic screening strategies, which are independent of known sequence information, can lead to the identification of truly novel genes and enzymes. Since E. coli has been used exhaustively for this purpose as a host, it is important to establish alternative expression hosts and to use...
Autores principales: | Leis, Benedikt, Angelov, Angel, Mientus, Markus, Li, Haijuan, Pham, Vu T. T., Lauinger, Benjamin, Bongen, Patrick, Pietruszka, Jörg, Gonçalves, Luís G., Santos, Helena, Liebl, Wolfgang |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389547/ https://www.ncbi.nlm.nih.gov/pubmed/25904908 http://dx.doi.org/10.3389/fmicb.2015.00275 |
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