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Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease

BACKGROUND: The polymorphisms in the components of the renin-angiotensin system (RAS) are important in the development and progression of coronary artery disease (CAD) in some individuals. Our objectives in the present investigation were to determine whether three RAS polymorphisms, angiotensin-conv...

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Autores principales: Al-Hazzani, Amal, Daoud, Mohamed S, Ataya, Farid S, Fouad, Dalia, Al-Jafari, Abdulaziz A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389886/
https://www.ncbi.nlm.nih.gov/pubmed/25984491
http://dx.doi.org/10.1186/2241-5793-21-8
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author Al-Hazzani, Amal
Daoud, Mohamed S
Ataya, Farid S
Fouad, Dalia
Al-Jafari, Abdulaziz A
author_facet Al-Hazzani, Amal
Daoud, Mohamed S
Ataya, Farid S
Fouad, Dalia
Al-Jafari, Abdulaziz A
author_sort Al-Hazzani, Amal
collection PubMed
description BACKGROUND: The polymorphisms in the components of the renin-angiotensin system (RAS) are important in the development and progression of coronary artery disease (CAD) in some individuals. Our objectives in the present investigation were to determine whether three RAS polymorphisms, angiotensin-converting enzyme insertion/deletion (ACE I/D), angiotensin receptor II (Ang II AT2 - C3123A) and angiotensinogen (AGT-M235T), are associated with CAD in the Saudi population. We recruited 225 subjects with angiographically confirmed CAD who had identical ethnic backgrounds and 110 control subjects. The polymerase chain reaction-restriction fragment length polymorphisms (RFLP) technique was used to detect polymorphisms in the RAS gene. RESULTS: Within the CAD group, for the ACE I/D genotype, DD was found in 64.4%, 26.3% carried the ID genotype, and 9.3% carried the II genotype. Within the control group, the DD genotype was found in 56.4%, 23.6% carried the ID genotype, and 20% carried the II genotype. The odds ratio (OR) of the ACE DD vs II genotype with a 95% confidence interval (CI) was 2.45 (1.26-4.78), with p = 0.008. For the Ang II AT2 receptor C3123A genotype, within the CAD group, CC was found in 39.6%, 17.8% carried the CA genotype, and 42.6% carried the AA genotype. Within the control group, CC was found in 39.1%, 60.9% carried the CA genotype, and there was an absence of the AA genotype. The OR of the Ang II AT2 receptor C3123A CC vs AA genotypes (95% CI) was 0.01, with p = 0.0001. A significant association with CAD was shown. For the AGT-M235T genotype, within the CAD group, MM was found in 24.0%, 43.6% carried the MT genotype and 32.4% carried the TT genotype. Within the control group, MM was found in 26.4%, 45.5% carried the TT genotype and 28.2% carried the MT genotype. The OR of MM vs TT (95% CI) was 0.79 (0.43 to 1.46), which was insignificant. CONCLUSIONS: There is an association between the ACE I/D and Ang II AT2 receptor C3123A polymorphisms and CAD, however, no association was detected between the AGT M235T polymorphism and CAD in the Saudi population.
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spelling pubmed-43898862015-05-15 Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease Al-Hazzani, Amal Daoud, Mohamed S Ataya, Farid S Fouad, Dalia Al-Jafari, Abdulaziz A J Biol Res (Thessalon) Research BACKGROUND: The polymorphisms in the components of the renin-angiotensin system (RAS) are important in the development and progression of coronary artery disease (CAD) in some individuals. Our objectives in the present investigation were to determine whether three RAS polymorphisms, angiotensin-converting enzyme insertion/deletion (ACE I/D), angiotensin receptor II (Ang II AT2 - C3123A) and angiotensinogen (AGT-M235T), are associated with CAD in the Saudi population. We recruited 225 subjects with angiographically confirmed CAD who had identical ethnic backgrounds and 110 control subjects. The polymerase chain reaction-restriction fragment length polymorphisms (RFLP) technique was used to detect polymorphisms in the RAS gene. RESULTS: Within the CAD group, for the ACE I/D genotype, DD was found in 64.4%, 26.3% carried the ID genotype, and 9.3% carried the II genotype. Within the control group, the DD genotype was found in 56.4%, 23.6% carried the ID genotype, and 20% carried the II genotype. The odds ratio (OR) of the ACE DD vs II genotype with a 95% confidence interval (CI) was 2.45 (1.26-4.78), with p = 0.008. For the Ang II AT2 receptor C3123A genotype, within the CAD group, CC was found in 39.6%, 17.8% carried the CA genotype, and 42.6% carried the AA genotype. Within the control group, CC was found in 39.1%, 60.9% carried the CA genotype, and there was an absence of the AA genotype. The OR of the Ang II AT2 receptor C3123A CC vs AA genotypes (95% CI) was 0.01, with p = 0.0001. A significant association with CAD was shown. For the AGT-M235T genotype, within the CAD group, MM was found in 24.0%, 43.6% carried the MT genotype and 32.4% carried the TT genotype. Within the control group, MM was found in 26.4%, 45.5% carried the TT genotype and 28.2% carried the MT genotype. The OR of MM vs TT (95% CI) was 0.79 (0.43 to 1.46), which was insignificant. CONCLUSIONS: There is an association between the ACE I/D and Ang II AT2 receptor C3123A polymorphisms and CAD, however, no association was detected between the AGT M235T polymorphism and CAD in the Saudi population. BioMed Central 2014-05-21 /pmc/articles/PMC4389886/ /pubmed/25984491 http://dx.doi.org/10.1186/2241-5793-21-8 Text en © Al-Hazzani et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Al-Hazzani, Amal
Daoud, Mohamed S
Ataya, Farid S
Fouad, Dalia
Al-Jafari, Abdulaziz A
Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease
title Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease
title_full Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease
title_fullStr Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease
title_full_unstemmed Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease
title_short Renin–angiotensin system gene polymorphisms among Saudi patients with coronary artery disease
title_sort renin–angiotensin system gene polymorphisms among saudi patients with coronary artery disease
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389886/
https://www.ncbi.nlm.nih.gov/pubmed/25984491
http://dx.doi.org/10.1186/2241-5793-21-8
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