Cargando…

In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons

BACKGROUND: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in...

Descripción completa

Detalles Bibliográficos
Autores principales: Tsialtas, Ioannis T, Patelou, Efstathia, Kaloumenos, Nikolaos S, Mylona, Photini V, Polidoros, Alexios, Menexes, Georgios, Eleftherohorinos, Ilias G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389997/
https://www.ncbi.nlm.nih.gov/pubmed/25984494
http://dx.doi.org/10.1186/2241-5793-21-11
_version_ 1782365635454435328
author Tsialtas, Ioannis T
Patelou, Efstathia
Kaloumenos, Nikolaos S
Mylona, Photini V
Polidoros, Alexios
Menexes, Georgios
Eleftherohorinos, Ilias G
author_facet Tsialtas, Ioannis T
Patelou, Efstathia
Kaloumenos, Nikolaos S
Mylona, Photini V
Polidoros, Alexios
Menexes, Georgios
Eleftherohorinos, Ilias G
author_sort Tsialtas, Ioannis T
collection PubMed
description BACKGROUND: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters. RESULTS: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids. CONCLUSIONS: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.
format Online
Article
Text
id pubmed-4389997
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-43899972015-05-15 In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons Tsialtas, Ioannis T Patelou, Efstathia Kaloumenos, Nikolaos S Mylona, Photini V Polidoros, Alexios Menexes, Georgios Eleftherohorinos, Ilias G J Biol Res (Thessalon) Research BACKGROUND: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters. RESULTS: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids. CONCLUSIONS: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions. BioMed Central 2014-06-23 /pmc/articles/PMC4389997/ /pubmed/25984494 http://dx.doi.org/10.1186/2241-5793-21-11 Text en © Tsialtas et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Tsialtas, Ioannis T
Patelou, Efstathia
Kaloumenos, Nikolaos S
Mylona, Photini V
Polidoros, Alexios
Menexes, Georgios
Eleftherohorinos, Ilias G
In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons
title In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons
title_full In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons
title_fullStr In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons
title_full_unstemmed In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons
title_short In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons
title_sort in the wild hybridization of annual datura species as unveiled by morphological and molecular comparisons
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389997/
https://www.ncbi.nlm.nih.gov/pubmed/25984494
http://dx.doi.org/10.1186/2241-5793-21-11
work_keys_str_mv AT tsialtasioannist inthewildhybridizationofannualdaturaspeciesasunveiledbymorphologicalandmolecularcomparisons
AT patelouefstathia inthewildhybridizationofannualdaturaspeciesasunveiledbymorphologicalandmolecularcomparisons
AT kaloumenosnikolaoss inthewildhybridizationofannualdaturaspeciesasunveiledbymorphologicalandmolecularcomparisons
AT mylonaphotiniv inthewildhybridizationofannualdaturaspeciesasunveiledbymorphologicalandmolecularcomparisons
AT polidorosalexios inthewildhybridizationofannualdaturaspeciesasunveiledbymorphologicalandmolecularcomparisons
AT menexesgeorgios inthewildhybridizationofannualdaturaspeciesasunveiledbymorphologicalandmolecularcomparisons
AT eleftherohorinosiliasg inthewildhybridizationofannualdaturaspeciesasunveiledbymorphologicalandmolecularcomparisons