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In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons
BACKGROUND: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389997/ https://www.ncbi.nlm.nih.gov/pubmed/25984494 http://dx.doi.org/10.1186/2241-5793-21-11 |
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author | Tsialtas, Ioannis T Patelou, Efstathia Kaloumenos, Nikolaos S Mylona, Photini V Polidoros, Alexios Menexes, Georgios Eleftherohorinos, Ilias G |
author_facet | Tsialtas, Ioannis T Patelou, Efstathia Kaloumenos, Nikolaos S Mylona, Photini V Polidoros, Alexios Menexes, Georgios Eleftherohorinos, Ilias G |
author_sort | Tsialtas, Ioannis T |
collection | PubMed |
description | BACKGROUND: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters. RESULTS: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids. CONCLUSIONS: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions. |
format | Online Article Text |
id | pubmed-4389997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43899972015-05-15 In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons Tsialtas, Ioannis T Patelou, Efstathia Kaloumenos, Nikolaos S Mylona, Photini V Polidoros, Alexios Menexes, Georgios Eleftherohorinos, Ilias G J Biol Res (Thessalon) Research BACKGROUND: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters. RESULTS: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids. CONCLUSIONS: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions. BioMed Central 2014-06-23 /pmc/articles/PMC4389997/ /pubmed/25984494 http://dx.doi.org/10.1186/2241-5793-21-11 Text en © Tsialtas et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Tsialtas, Ioannis T Patelou, Efstathia Kaloumenos, Nikolaos S Mylona, Photini V Polidoros, Alexios Menexes, Georgios Eleftherohorinos, Ilias G In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons |
title | In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons |
title_full | In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons |
title_fullStr | In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons |
title_full_unstemmed | In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons |
title_short | In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons |
title_sort | in the wild hybridization of annual datura species as unveiled by morphological and molecular comparisons |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4389997/ https://www.ncbi.nlm.nih.gov/pubmed/25984494 http://dx.doi.org/10.1186/2241-5793-21-11 |
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