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Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro

BACKGROUND: This study was performed to describe the basic methods to isolate and culture of primary satellite cells (PSCs) obtained from 50 to 60-day-old sheep fetuses, single cell cloning of transfected PSCs and sexing of ovine PSCs based on the ZFY/ZFX, amelogenin and high-motility-group (HMG) bo...

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Detalles Bibliográficos
Autores principales:	Salabi, Fatemeh, Nazari, Mahmood, Cao, Wen G
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390094/ https://www.ncbi.nlm.nih.gov/pubmed/25984504 http://dx.doi.org/10.1186/s40709-014-0022-z

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