Cargando…

IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model

BACKGROUND: Th2-promoting cytokine IL-25 might contribute to bronchial mucosal vascular remodelling in asthma through its receptor expressed by vascular endothelial and vascular smooth muscle cells. METHODS: By utilising a newly established chronic asthma murine model induced by direct exposure of t...

Descripción completa

Detalles Bibliográficos
Autores principales: Yao, Xiujuan, Wang, Wei, Li, Yan, Huang, Ping, Zhang, Qian, Wang, Jingjing, Wang, Wen, Lv, Zhe, An, Yunqing, Qin, Jianguo, Corrigan, Chris J, Huang, Kewu, Sun, Yongchang, Ying, Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390095/
https://www.ncbi.nlm.nih.gov/pubmed/25889697
http://dx.doi.org/10.1186/s12931-015-0197-3
_version_ 1782365640814755840
author Yao, Xiujuan
Wang, Wei
Li, Yan
Huang, Ping
Zhang, Qian
Wang, Jingjing
Wang, Wen
Lv, Zhe
An, Yunqing
Qin, Jianguo
Corrigan, Chris J
Huang, Kewu
Sun, Yongchang
Ying, Sun
author_facet Yao, Xiujuan
Wang, Wei
Li, Yan
Huang, Ping
Zhang, Qian
Wang, Jingjing
Wang, Wen
Lv, Zhe
An, Yunqing
Qin, Jianguo
Corrigan, Chris J
Huang, Kewu
Sun, Yongchang
Ying, Sun
author_sort Yao, Xiujuan
collection PubMed
description BACKGROUND: Th2-promoting cytokine IL-25 might contribute to bronchial mucosal vascular remodelling in asthma through its receptor expressed by vascular endothelial and vascular smooth muscle cells. METHODS: By utilising a newly established chronic asthma murine model induced by direct exposure of the airways to IL-25 alone, we examined effects of IL-25 on angiogenesis, vascular remodelling and expression of angiogenic factors, compared changes with those in a “classical” ovalbumin (OVA)-induced murine asthma model. IL-25 and OVA were intranasally instilled into the airways of BALB/c mice for up to 55 days. Airways vessels and angiogenic factors, including Von Willebrand Factor (vWF), amphiregulin, angiogenin, endothelin-1, transcription factor ERG, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin-like growth factor (IGF-1) and vascular endothelial growth factor (VEGF) in lung sections, homogenates and BAL fluid were detected and quantified by immunostaining or enzyme linked immunosorbent assay (ELISA). An in house assay was also utilised to compare the effects of IL-25 and other Th2-cytokines on angiogenesis by human vascular endothelial cells. RESULTS: Repetitive intranasal challenge with IL-25 alone or OVA alone in OVA-presensitised animals significantly increased peribronchial vWF (+) vessels in the murine airways, which was associated with remarkably elevated expression of amphiregulin, angiogenin, endothelin-1, bFGF, EGF, IGF-1, VEGF and ERG. IL-25, but not Th-2-cytokines induced human angiogenesis in vitro. CONCLUSIONS: The data suggest that chronic exposure of murine airways to IL-25 alone is able to reproduce a local angiogenic milieu. Thus, blocking IL-25 may attenuate vascular remodelling and improve outcomes in asthma patients.
format Online
Article
Text
id pubmed-4390095
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-43900952015-04-09 IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model Yao, Xiujuan Wang, Wei Li, Yan Huang, Ping Zhang, Qian Wang, Jingjing Wang, Wen Lv, Zhe An, Yunqing Qin, Jianguo Corrigan, Chris J Huang, Kewu Sun, Yongchang Ying, Sun Respir Res Research BACKGROUND: Th2-promoting cytokine IL-25 might contribute to bronchial mucosal vascular remodelling in asthma through its receptor expressed by vascular endothelial and vascular smooth muscle cells. METHODS: By utilising a newly established chronic asthma murine model induced by direct exposure of the airways to IL-25 alone, we examined effects of IL-25 on angiogenesis, vascular remodelling and expression of angiogenic factors, compared changes with those in a “classical” ovalbumin (OVA)-induced murine asthma model. IL-25 and OVA were intranasally instilled into the airways of BALB/c mice for up to 55 days. Airways vessels and angiogenic factors, including Von Willebrand Factor (vWF), amphiregulin, angiogenin, endothelin-1, transcription factor ERG, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin-like growth factor (IGF-1) and vascular endothelial growth factor (VEGF) in lung sections, homogenates and BAL fluid were detected and quantified by immunostaining or enzyme linked immunosorbent assay (ELISA). An in house assay was also utilised to compare the effects of IL-25 and other Th2-cytokines on angiogenesis by human vascular endothelial cells. RESULTS: Repetitive intranasal challenge with IL-25 alone or OVA alone in OVA-presensitised animals significantly increased peribronchial vWF (+) vessels in the murine airways, which was associated with remarkably elevated expression of amphiregulin, angiogenin, endothelin-1, bFGF, EGF, IGF-1, VEGF and ERG. IL-25, but not Th-2-cytokines induced human angiogenesis in vitro. CONCLUSIONS: The data suggest that chronic exposure of murine airways to IL-25 alone is able to reproduce a local angiogenic milieu. Thus, blocking IL-25 may attenuate vascular remodelling and improve outcomes in asthma patients. BioMed Central 2015-03-18 2015 /pmc/articles/PMC4390095/ /pubmed/25889697 http://dx.doi.org/10.1186/s12931-015-0197-3 Text en © Yao et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Yao, Xiujuan
Wang, Wei
Li, Yan
Huang, Ping
Zhang, Qian
Wang, Jingjing
Wang, Wen
Lv, Zhe
An, Yunqing
Qin, Jianguo
Corrigan, Chris J
Huang, Kewu
Sun, Yongchang
Ying, Sun
IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
title IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
title_full IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
title_fullStr IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
title_full_unstemmed IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
title_short IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
title_sort il-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390095/
https://www.ncbi.nlm.nih.gov/pubmed/25889697
http://dx.doi.org/10.1186/s12931-015-0197-3
work_keys_str_mv AT yaoxiujuan il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT wangwei il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT liyan il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT huangping il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT zhangqian il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT wangjingjing il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT wangwen il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT lvzhe il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT anyunqing il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT qinjianguo il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT corriganchrisj il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT huangkewu il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT sunyongchang il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel
AT yingsun il25inducesairwaysangiogenesisandexpressionofmultipleangiogenicfactorsinamurineasthmamodel