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IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model
BACKGROUND: Th2-promoting cytokine IL-25 might contribute to bronchial mucosal vascular remodelling in asthma through its receptor expressed by vascular endothelial and vascular smooth muscle cells. METHODS: By utilising a newly established chronic asthma murine model induced by direct exposure of t...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390095/ https://www.ncbi.nlm.nih.gov/pubmed/25889697 http://dx.doi.org/10.1186/s12931-015-0197-3 |
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author | Yao, Xiujuan Wang, Wei Li, Yan Huang, Ping Zhang, Qian Wang, Jingjing Wang, Wen Lv, Zhe An, Yunqing Qin, Jianguo Corrigan, Chris J Huang, Kewu Sun, Yongchang Ying, Sun |
author_facet | Yao, Xiujuan Wang, Wei Li, Yan Huang, Ping Zhang, Qian Wang, Jingjing Wang, Wen Lv, Zhe An, Yunqing Qin, Jianguo Corrigan, Chris J Huang, Kewu Sun, Yongchang Ying, Sun |
author_sort | Yao, Xiujuan |
collection | PubMed |
description | BACKGROUND: Th2-promoting cytokine IL-25 might contribute to bronchial mucosal vascular remodelling in asthma through its receptor expressed by vascular endothelial and vascular smooth muscle cells. METHODS: By utilising a newly established chronic asthma murine model induced by direct exposure of the airways to IL-25 alone, we examined effects of IL-25 on angiogenesis, vascular remodelling and expression of angiogenic factors, compared changes with those in a “classical” ovalbumin (OVA)-induced murine asthma model. IL-25 and OVA were intranasally instilled into the airways of BALB/c mice for up to 55 days. Airways vessels and angiogenic factors, including Von Willebrand Factor (vWF), amphiregulin, angiogenin, endothelin-1, transcription factor ERG, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin-like growth factor (IGF-1) and vascular endothelial growth factor (VEGF) in lung sections, homogenates and BAL fluid were detected and quantified by immunostaining or enzyme linked immunosorbent assay (ELISA). An in house assay was also utilised to compare the effects of IL-25 and other Th2-cytokines on angiogenesis by human vascular endothelial cells. RESULTS: Repetitive intranasal challenge with IL-25 alone or OVA alone in OVA-presensitised animals significantly increased peribronchial vWF (+) vessels in the murine airways, which was associated with remarkably elevated expression of amphiregulin, angiogenin, endothelin-1, bFGF, EGF, IGF-1, VEGF and ERG. IL-25, but not Th-2-cytokines induced human angiogenesis in vitro. CONCLUSIONS: The data suggest that chronic exposure of murine airways to IL-25 alone is able to reproduce a local angiogenic milieu. Thus, blocking IL-25 may attenuate vascular remodelling and improve outcomes in asthma patients. |
format | Online Article Text |
id | pubmed-4390095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43900952015-04-09 IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model Yao, Xiujuan Wang, Wei Li, Yan Huang, Ping Zhang, Qian Wang, Jingjing Wang, Wen Lv, Zhe An, Yunqing Qin, Jianguo Corrigan, Chris J Huang, Kewu Sun, Yongchang Ying, Sun Respir Res Research BACKGROUND: Th2-promoting cytokine IL-25 might contribute to bronchial mucosal vascular remodelling in asthma through its receptor expressed by vascular endothelial and vascular smooth muscle cells. METHODS: By utilising a newly established chronic asthma murine model induced by direct exposure of the airways to IL-25 alone, we examined effects of IL-25 on angiogenesis, vascular remodelling and expression of angiogenic factors, compared changes with those in a “classical” ovalbumin (OVA)-induced murine asthma model. IL-25 and OVA were intranasally instilled into the airways of BALB/c mice for up to 55 days. Airways vessels and angiogenic factors, including Von Willebrand Factor (vWF), amphiregulin, angiogenin, endothelin-1, transcription factor ERG, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), insulin-like growth factor (IGF-1) and vascular endothelial growth factor (VEGF) in lung sections, homogenates and BAL fluid were detected and quantified by immunostaining or enzyme linked immunosorbent assay (ELISA). An in house assay was also utilised to compare the effects of IL-25 and other Th2-cytokines on angiogenesis by human vascular endothelial cells. RESULTS: Repetitive intranasal challenge with IL-25 alone or OVA alone in OVA-presensitised animals significantly increased peribronchial vWF (+) vessels in the murine airways, which was associated with remarkably elevated expression of amphiregulin, angiogenin, endothelin-1, bFGF, EGF, IGF-1, VEGF and ERG. IL-25, but not Th-2-cytokines induced human angiogenesis in vitro. CONCLUSIONS: The data suggest that chronic exposure of murine airways to IL-25 alone is able to reproduce a local angiogenic milieu. Thus, blocking IL-25 may attenuate vascular remodelling and improve outcomes in asthma patients. BioMed Central 2015-03-18 2015 /pmc/articles/PMC4390095/ /pubmed/25889697 http://dx.doi.org/10.1186/s12931-015-0197-3 Text en © Yao et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Yao, Xiujuan Wang, Wei Li, Yan Huang, Ping Zhang, Qian Wang, Jingjing Wang, Wen Lv, Zhe An, Yunqing Qin, Jianguo Corrigan, Chris J Huang, Kewu Sun, Yongchang Ying, Sun IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model |
title | IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model |
title_full | IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model |
title_fullStr | IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model |
title_full_unstemmed | IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model |
title_short | IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model |
title_sort | il-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390095/ https://www.ncbi.nlm.nih.gov/pubmed/25889697 http://dx.doi.org/10.1186/s12931-015-0197-3 |
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