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European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize
We have investigated the role of selection in the determination of the detected levels of introgression from modern maize hybrid varieties into maize landraces still cultivated in situ in Italy. We exploited the availability of a historical collection of landraces undertaken before the introduction...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390310/ https://www.ncbi.nlm.nih.gov/pubmed/25853809 http://dx.doi.org/10.1371/journal.pone.0121381 |
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author | Bitocchi, Elena Bellucci, Elisa Rau, Domenico Albertini, Emidio Rodriguez, Monica Veronesi, Fabio Attene, Giovanna Nanni, Laura |
author_facet | Bitocchi, Elena Bellucci, Elisa Rau, Domenico Albertini, Emidio Rodriguez, Monica Veronesi, Fabio Attene, Giovanna Nanni, Laura |
author_sort | Bitocchi, Elena |
collection | PubMed |
description | We have investigated the role of selection in the determination of the detected levels of introgression from modern maize hybrid varieties into maize landraces still cultivated in situ in Italy. We exploited the availability of a historical collection of landraces undertaken before the introduction and widespread use of modern maize, to analyse genomic changes that have occurred in these maize landraces over 50 years of co-existence with hybrid varieties. We have combined a previously published SSR dataset (n=21) with an AFLP loci dataset (n=168) to provide higher resolution power and to obtain a more detailed picture. We show that selection pressures for adaptation have favoured new alleles introduced by migration from hybrids. This shows the potential for analysis of historical introgression even over this short period of 50 years, for an understanding of the evolution of the genome and for the identification of its functionally important regions. Moreover, this demonstrates that landraces grown in situ represent almost unique populations for use for such studies when the focus is on the domesticated plant. This is due to their adaptation, which has arisen from their dynamic evolution under a continuously changing agro-ecological environment, and their capture of new alleles from hybridisation. We have also identified loci for which selection has inhibited introgression from modern germplasm and has enhanced the distinction between landraces and modern maize. These loci indicate that selection acted in the past, during the formation of the flint and dent gene pools. In particular, the locus showing the strongest signals of selection is a Misfit transposable element. Finally, molecular characterisation of the same samples with two different molecular markers has allowed us to compare their performances. Although the genetic-diversity and population-structure analyses provide the same global qualitative pattern, which thus provides the same inferences, there are differences related to their natures and characteristics. |
format | Online Article Text |
id | pubmed-4390310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43903102015-04-21 European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize Bitocchi, Elena Bellucci, Elisa Rau, Domenico Albertini, Emidio Rodriguez, Monica Veronesi, Fabio Attene, Giovanna Nanni, Laura PLoS One Research Article We have investigated the role of selection in the determination of the detected levels of introgression from modern maize hybrid varieties into maize landraces still cultivated in situ in Italy. We exploited the availability of a historical collection of landraces undertaken before the introduction and widespread use of modern maize, to analyse genomic changes that have occurred in these maize landraces over 50 years of co-existence with hybrid varieties. We have combined a previously published SSR dataset (n=21) with an AFLP loci dataset (n=168) to provide higher resolution power and to obtain a more detailed picture. We show that selection pressures for adaptation have favoured new alleles introduced by migration from hybrids. This shows the potential for analysis of historical introgression even over this short period of 50 years, for an understanding of the evolution of the genome and for the identification of its functionally important regions. Moreover, this demonstrates that landraces grown in situ represent almost unique populations for use for such studies when the focus is on the domesticated plant. This is due to their adaptation, which has arisen from their dynamic evolution under a continuously changing agro-ecological environment, and their capture of new alleles from hybridisation. We have also identified loci for which selection has inhibited introgression from modern germplasm and has enhanced the distinction between landraces and modern maize. These loci indicate that selection acted in the past, during the formation of the flint and dent gene pools. In particular, the locus showing the strongest signals of selection is a Misfit transposable element. Finally, molecular characterisation of the same samples with two different molecular markers has allowed us to compare their performances. Although the genetic-diversity and population-structure analyses provide the same global qualitative pattern, which thus provides the same inferences, there are differences related to their natures and characteristics. Public Library of Science 2015-04-08 /pmc/articles/PMC4390310/ /pubmed/25853809 http://dx.doi.org/10.1371/journal.pone.0121381 Text en © 2015 Bitocchi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bitocchi, Elena Bellucci, Elisa Rau, Domenico Albertini, Emidio Rodriguez, Monica Veronesi, Fabio Attene, Giovanna Nanni, Laura European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize |
title | European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize |
title_full | European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize |
title_fullStr | European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize |
title_full_unstemmed | European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize |
title_short | European Flint Landraces Grown In Situ Reveal Adaptive Introgression from Modern Maize |
title_sort | european flint landraces grown in situ reveal adaptive introgression from modern maize |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390310/ https://www.ncbi.nlm.nih.gov/pubmed/25853809 http://dx.doi.org/10.1371/journal.pone.0121381 |
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