Cargando…

Multiplex Real-Time PCR Method for Simultaneous Identification and Toxigenic Type Characterization of Clostridium difficile From Stool Samples

BACKGROUND: The aim of this study was to develop and validate a multiplex real-time PCR assay for simultaneous identification and toxigenic type characterization of Clostridium difficile. METHODS: The multiplex real-time PCR assay targeted and simultaneously detected triose phosphate isomerase (tpi)...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kilic, Abdullah, Alam, Mohammad J., Tisdel, Naradah L., Shah, Dhara N., Yapar, Mehmet, Lasco, Todd M., Garey, Kevin W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Korean Society for Laboratory Medicine 2015
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390698/ https://www.ncbi.nlm.nih.gov/pubmed/25932438 http://dx.doi.org/10.3343/alm.2015.35.3.306

Ejemplares similares

Community Environmental Contamination of Toxigenic Clostridium difficile
por: Alam, M Jahangir, et al.
Publicado: (2017)

Development of TaqMan-Based Quantitative PCR for Sensitive and Selective Detection of Toxigenic Clostridium difficile in Human Stools
por: Kubota, Hiroyuki, et al.
Publicado: (2014)

Clinical importance and representation of toxigenic and non-toxigenic Clostridium difficile cultivated from stool samples of hospitalized patients
por: Predrag, Stojanovic, et al.
Publicado: (2012)

510. First Environmental Investigation of Toxigenic Clostridium difficile Strains in Texas Hospitals
por: Alam, M Jahangir, et al.
Publicado: (2018)

Diagnosis of Clostridium difficile infection by toxigenic culture and PCR assay
por: Mirzaei, Elnaze Zare, et al.
Publicado: (2018)

First Environmental Investigation of Toxigenic Clostridium difficile at a Large Hospital in Bangladesh
por: Alam, M Jahangir, et al.
Publicado: (2017)

Rate of Detection of Multiple Organisms and Clostridium difficile with Stool Multiplex PCR Detection Test in Pediatrics
por: Mangla, Saisho, et al.
Publicado: (2017)

1673 Microbial and Inflammatory Markers for Fatal Clostridium difficile Associated Diarrhea
por: Jiang, Zhi-Dong, et al.
Publicado: (2014)

Using Dog Scent Detection as a Point-of-Care Tool to Identify Toxigenic Clostridium difficile in Stool
por: Taylor, Maureen T, et al.
Publicado: (2018)

Evaluation of Multiplex PCR with Enhanced Spore Germination for Detection of Clostridium difficile from Stool Samples of the Hospitalized Patients
por: Chankhamhaengdecha, Surang, et al.
Publicado: (2013)

Healthcare Resource Utilization for Recurrent Clostridium difficile Infection in a Large University Hospital in Houston, Texas
por: Aitken, Samuel L., et al.
Publicado: (2014)

Isolation of toxigenic Clostridium difficile from ready-to-eat salads by multiplex polymerase chain reaction in Isfahan, Iran
por: Yamoudy, Mahire, et al.
Publicado: (2015)

Protective Effects of Bifidobacterial Strains Against Toxigenic Clostridium difficile
por: Wei, Yanxia, et al.
Publicado: (2018)

Clostridium difficile Enteritis With NAP7/078 Toxigenic Strain
por: Zewude, Rahel T, et al.
Publicado: (2023)

Evaluation of Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile Assays for Direct Detection of Toxigenic Clostridium difficile in Stool Specimens
por: Shin, Bo-Moon, et al.
Publicado: (2016)

Diagnostic yield of repeat sampling with immunoassay, real-time PCR, and toxigenic culture for the detection of toxigenic Clostridium difficile in an epidemic and a non-epidemic setting
por: van Prehn, J., et al.
Publicado: (2015)

710. Increased Clinical Failure Rates Associated with Reduced Metronidazole Susceptibility in Clostridioides difficile
por: Gonzales-Luna, Anne, et al.
Publicado: (2018)

A rapid multiplex real-time PCR detection of toxigenic Clostridioides difficile directly from fecal samples
por: Jia, Xiao-xi, et al.
Publicado: (2023)

Absence of Clostridium difficile stool carriage in asymptomatic volunteers
por: Hell, M, et al.
Publicado: (2011)

Impacts of infection with different toxigenic Clostridium difficile strains on faecal microbiota in children
por: Ling, Zongxin, et al.
Publicado: (2014)

High prevalence of toxigenic Clostridium difficile in public space lawns in Western Australia
por: Moono, Peter, et al.
Publicado: (2017)

Colonization of toxigenic Clostridium difficile among ICU patients: a prospective study
por: Zhang, Xiaoxia, et al.
Publicado: (2016)

Prevalence of Clostridium difficile and its toxigenic genotype in beef samples in west of Iran
por: Kheradmand, Malihe, et al.
Publicado: (2017)

Prevalence and antimicrobial susceptibility pattern of toxigenic Clostridium difficile strains isolated in Iran
por: MOHAMMADBEIGI, Maryam, et al.
Publicado: (2019)

Clinical Significance of Toxigenic Clostridioides difficile Growth in Stool Cultures during the Era of Nonculture Methods for the Diagnosis of C. difficile Infection
por: Lee, Ching-Chi, et al.
Publicado: (2021)

Comparison of a frozen human foreskin fibroblast cell assay to an enzyme immunoassay and toxigenic culture for the detection of toxigenic Clostridium difficile()()()
por: Strachan, Alastair J., et al.
Publicado: (2013)

1626 The relationship of Bristol Stool Scale to Clostridium difficile infection
por: Caroff, Daniel, et al.
Publicado: (2014)

Horizontal gene transfer converts non-toxigenic Clostridium difficile strains into toxin producers
por: Brouwer, Michael S.M., et al.
Publicado: (2013)

Longitudinal investigation of carriage rates and genotypes of toxigenic Clostridium difficile in hepatic cirrhosis patients
por: Chen, Yunbo, et al.
Publicado: (2019)

Toxigenic Clostridium difficile colonization among hospitalised adults; risk factors and impact on survival
por: Behar, Laura, et al.
Publicado: (2017)

Enterococcus faecalis Isolated From Infant Feces Inhibits Toxigenic Clostridioides (Clostridium) difficile
por: Romyasamit, Chonticha, et al.
Publicado: (2020)

Risk Factors of Fecal Toxigenic or Non-Toxigenic Clostridium difficile Colonization: Impact of Toll-Like Receptor Polymorphisms and Prior Antibiotic Exposure
por: Hung, Yuan-Pin, et al.
Publicado: (2013)

Design and validation of a multiplex PCR method for the simultaneous quantification of Clostridium acetobutylicum, Clostridium carboxidivorans and Clostridium cellulovorans
por: Feliu-Paradeda, Laura, et al.
Publicado: (2023)

Clostridium difficile PCR Ribotypes in Calves, Canada
por: Rodriguez-Palacios, Alexander, et al.
Publicado: (2006)

Performance of commercial PCR assays to detect toxigenic Clostridioides difficile in the feces of puppies
por: Anis, Eman, et al.
Publicado: (2021)

Predominance of Clostridium difficile Ribotypes 017 and 078 among Toxigenic Clinical Isolates in Southern Taiwan
por: Hung, Yuan-Pin, et al.
Publicado: (2016)

Detection of toxigenic Clostridium difficile colonization in patients admitted to the hospital for chemotherapy or haematopoietic cell transplantation
por: Vaughn, John L., et al.
Publicado: (2018)

Clinical and microbial characterization of toxigenic Clostridium difficile isolated from antibiotic associated diarrhea in Egypt
por: Elgendy, Sherein G., et al.
Publicado: (2020)

Toxin profiles and antimicrobial resistance patterns among toxigenic clinical isolates of Clostridioides (Clostridium) difficile
por: Heidari, Hamid, et al.
Publicado: (2019)

A prospective study of two isothermal amplification assays compared with real-time PCR, CCNA and toxigenic culture for the diagnosis of Clostridium difficile infection
por: Neuendorf, Martina, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_57jsn5nr3ksr8jo99o92qhbadc