Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?

We report the spontaneous generation of a cell-like morphology in an environment crowded with the polymers dextran and polyethylene glycol (PEG) in the presence of DNA. DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase...

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Autores principales: Tsumoto, Kanta, Arai, Masafumi, Nakatani, Naoki, Watanabe, Shun N., Yoshikawa, Kenichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Short Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390863/
https://www.ncbi.nlm.nih.gov/pubmed/25809964
http://dx.doi.org/10.3390/life5010459
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author Tsumoto, Kanta
Arai, Masafumi
Nakatani, Naoki
Watanabe, Shun N.
Yoshikawa, Kenichi
author_facet Tsumoto, Kanta
Arai, Masafumi
Nakatani, Naoki
Watanabe, Shun N.
Yoshikawa, Kenichi
author_sort Tsumoto, Kanta
collection PubMed
description We report the spontaneous generation of a cell-like morphology in an environment crowded with the polymers dextran and polyethylene glycol (PEG) in the presence of DNA. DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation. The resulting micro-droplets could be controlled by the use of optical tweezers. As an example of laser manipulation, the dynamic fusion of two droplets is reported, which resembles the process of cell division in time-reverse. A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed.
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spelling pubmed-43908632015-05-21 Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer? Tsumoto, Kanta Arai, Masafumi Nakatani, Naoki Watanabe, Shun N. Yoshikawa, Kenichi Life (Basel) Short Note We report the spontaneous generation of a cell-like morphology in an environment crowded with the polymers dextran and polyethylene glycol (PEG) in the presence of DNA. DNA molecules were selectively located in the interior of dextran-rich micro-droplets, when the composition of an aqueous two-phase system (ATPS) was near the critical condition of phase-segregation. The resulting micro-droplets could be controlled by the use of optical tweezers. As an example of laser manipulation, the dynamic fusion of two droplets is reported, which resembles the process of cell division in time-reverse. A hypothetical scenario for the emergence of a primitive cell with DNA is briefly discussed. MDPI 2015-02-09 /pmc/articles/PMC4390863/ /pubmed/25809964 http://dx.doi.org/10.3390/life5010459 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Note
Tsumoto, Kanta
Arai, Masafumi
Nakatani, Naoki
Watanabe, Shun N.
Yoshikawa, Kenichi
Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?
title Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?
title_full Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?
title_fullStr Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?
title_full_unstemmed Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?
title_short Does DNA Exert an Active Role in Generating Cell-Sized Spheres in an Aqueous Solution with a Crowding Binary Polymer?
title_sort does dna exert an active role in generating cell-sized spheres in an aqueous solution with a crowding binary polymer?
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390863/
https://www.ncbi.nlm.nih.gov/pubmed/25809964
http://dx.doi.org/10.3390/life5010459
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