Cargando…

pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements

Plasmids occur frequently in Archaea. A novel plasmid (denoted pTC1) containing typical conjugation functions has been isolated from Sulfolobus tengchongensis RT8-4, a strain obtained from a hot spring in Tengchong, China, and characterized. The plasmid is a circular double-stranded DNA molecule of...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiang, Xiaoyu, Huang, Xiaoxing, Wang, Haina, Huang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390865/
https://www.ncbi.nlm.nih.gov/pubmed/25686154
http://dx.doi.org/10.3390/life5010506
_version_ 1782365740588859392
author Xiang, Xiaoyu
Huang, Xiaoxing
Wang, Haina
Huang, Li
author_facet Xiang, Xiaoyu
Huang, Xiaoxing
Wang, Haina
Huang, Li
author_sort Xiang, Xiaoyu
collection PubMed
description Plasmids occur frequently in Archaea. A novel plasmid (denoted pTC1) containing typical conjugation functions has been isolated from Sulfolobus tengchongensis RT8-4, a strain obtained from a hot spring in Tengchong, China, and characterized. The plasmid is a circular double-stranded DNA molecule of 20,417 bp. Among a total of 26 predicted pTC1 ORFs, 23 have homologues in other known Sulfolobus conjugative plasmids (CPs). pTC1 resembles other Sulfolobus CPs in genome architecture, and is most highly conserved in the genomic region encoding conjugation functions. However, attempts to demonstrate experimentally the capacity of the plasmid for conjugational transfer were unsuccessful. A survey revealed that pTC1 and its closely related plasmid variants were widespread in the geothermal area of Tengchong. Variations of the plasmids at the target sites for transposition by an insertion sequence (IS) and a miniature inverted-repeat transposable element (MITE) were readily detected. The IS was efficiently inserted into the pTC1 genome, and the inserted sequence was inactivated and degraded more frequently in an imprecise manner than in a precise manner. These results suggest that the host organism has evolved a strategy to maintain a balance between the insertion and elimination of mobile genetic elements to permit genomic plasticity while inhibiting their fast spreading.
format Online
Article
Text
id pubmed-4390865
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-43908652015-05-21 pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements Xiang, Xiaoyu Huang, Xiaoxing Wang, Haina Huang, Li Life (Basel) Article Plasmids occur frequently in Archaea. A novel plasmid (denoted pTC1) containing typical conjugation functions has been isolated from Sulfolobus tengchongensis RT8-4, a strain obtained from a hot spring in Tengchong, China, and characterized. The plasmid is a circular double-stranded DNA molecule of 20,417 bp. Among a total of 26 predicted pTC1 ORFs, 23 have homologues in other known Sulfolobus conjugative plasmids (CPs). pTC1 resembles other Sulfolobus CPs in genome architecture, and is most highly conserved in the genomic region encoding conjugation functions. However, attempts to demonstrate experimentally the capacity of the plasmid for conjugational transfer were unsuccessful. A survey revealed that pTC1 and its closely related plasmid variants were widespread in the geothermal area of Tengchong. Variations of the plasmids at the target sites for transposition by an insertion sequence (IS) and a miniature inverted-repeat transposable element (MITE) were readily detected. The IS was efficiently inserted into the pTC1 genome, and the inserted sequence was inactivated and degraded more frequently in an imprecise manner than in a precise manner. These results suggest that the host organism has evolved a strategy to maintain a balance between the insertion and elimination of mobile genetic elements to permit genomic plasticity while inhibiting their fast spreading. MDPI 2015-02-12 /pmc/articles/PMC4390865/ /pubmed/25686154 http://dx.doi.org/10.3390/life5010506 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiang, Xiaoyu
Huang, Xiaoxing
Wang, Haina
Huang, Li
pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements
title pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements
title_full pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements
title_fullStr pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements
title_full_unstemmed pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements
title_short pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements
title_sort ptc plasmids from sulfolobus species in the geothermal area of tengchong, china: genomic conservation and naturally-occurring variations as a result of transposition by mobile genetic elements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390865/
https://www.ncbi.nlm.nih.gov/pubmed/25686154
http://dx.doi.org/10.3390/life5010506
work_keys_str_mv AT xiangxiaoyu ptcplasmidsfromsulfolobusspeciesinthegeothermalareaoftengchongchinagenomicconservationandnaturallyoccurringvariationsasaresultoftranspositionbymobilegeneticelements
AT huangxiaoxing ptcplasmidsfromsulfolobusspeciesinthegeothermalareaoftengchongchinagenomicconservationandnaturallyoccurringvariationsasaresultoftranspositionbymobilegeneticelements
AT wanghaina ptcplasmidsfromsulfolobusspeciesinthegeothermalareaoftengchongchinagenomicconservationandnaturallyoccurringvariationsasaresultoftranspositionbymobilegeneticelements
AT huangli ptcplasmidsfromsulfolobusspeciesinthegeothermalareaoftengchongchinagenomicconservationandnaturallyoccurringvariationsasaresultoftranspositionbymobilegeneticelements