Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy

Cladophora glomerata extract (CGE) has been shown to exhibit antigastric ulcer, anti-inflammatory, analgesic, hypotensive, and antioxidant activities. The present study investigated antidiabetic and renoprotective effects of CGE in type 2 diabetes mellitus (T2DM) rats. The rats were induced by high-...

Descripción completa

Detalles Bibliográficos
Autores principales: Srimaroeng, Chutima, Ontawong, Atcharaporn, Saowakon, Naruwan, Vivithanaporn, Pornpun, Pongchaidecha, Anchalee, Amornlerdpison, Doungporn, Soodvilai, Sunhapas, Chatsudthipong, Varanuj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391723/
https://www.ncbi.nlm.nih.gov/pubmed/25883984
http://dx.doi.org/10.1155/2015/320167
_version_ 1782365866082435072
author Srimaroeng, Chutima
Ontawong, Atcharaporn
Saowakon, Naruwan
Vivithanaporn, Pornpun
Pongchaidecha, Anchalee
Amornlerdpison, Doungporn
Soodvilai, Sunhapas
Chatsudthipong, Varanuj
author_facet Srimaroeng, Chutima
Ontawong, Atcharaporn
Saowakon, Naruwan
Vivithanaporn, Pornpun
Pongchaidecha, Anchalee
Amornlerdpison, Doungporn
Soodvilai, Sunhapas
Chatsudthipong, Varanuj
author_sort Srimaroeng, Chutima
collection PubMed
description Cladophora glomerata extract (CGE) has been shown to exhibit antigastric ulcer, anti-inflammatory, analgesic, hypotensive, and antioxidant activities. The present study investigated antidiabetic and renoprotective effects of CGE in type 2 diabetes mellitus (T2DM) rats. The rats were induced by high-fat diet and streptozotocin and supplemented daily with 1 g/kg BW of CGE for 12 weeks. The renal transport function was assessed by the uptake of para-aminohippurate mediated organic anion transporters 1 (Oat1) and 3 (Oat3), using renal cortical slices. These two transporters were known to be upregulated by insulin and PKCζ while they were downregulated by PKCα activation. Compared to T2DM, CGE supplemented rats had significantly improved hyperglycaemia, hypertriglyceridemia, insulin resistance, and renal morphology. The baseline uptake of para-aminohippurate was not different among experimental groups and was correlated with Oat1 and 3 mRNA expressions. Nevertheless, while insulin-stimulated Oat1 and 3 functions in renal slices were blunted in T2DM rats, they were improved by CGE supplementation. The mechanism of CGE-restored insulin-stimulated Oat1 and 3 functions was clearly shown to be associated with upregulated PKCζ and downregulated PKCα expressions and activations. These findings indicate that CGE has antidiabetic effect and suggest it may prevent diabetic nephropathy through PKCs in a T2DM rat model.
format Online
Article
Text
id pubmed-4391723
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-43917232015-04-16 Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy Srimaroeng, Chutima Ontawong, Atcharaporn Saowakon, Naruwan Vivithanaporn, Pornpun Pongchaidecha, Anchalee Amornlerdpison, Doungporn Soodvilai, Sunhapas Chatsudthipong, Varanuj J Diabetes Res Research Article Cladophora glomerata extract (CGE) has been shown to exhibit antigastric ulcer, anti-inflammatory, analgesic, hypotensive, and antioxidant activities. The present study investigated antidiabetic and renoprotective effects of CGE in type 2 diabetes mellitus (T2DM) rats. The rats were induced by high-fat diet and streptozotocin and supplemented daily with 1 g/kg BW of CGE for 12 weeks. The renal transport function was assessed by the uptake of para-aminohippurate mediated organic anion transporters 1 (Oat1) and 3 (Oat3), using renal cortical slices. These two transporters were known to be upregulated by insulin and PKCζ while they were downregulated by PKCα activation. Compared to T2DM, CGE supplemented rats had significantly improved hyperglycaemia, hypertriglyceridemia, insulin resistance, and renal morphology. The baseline uptake of para-aminohippurate was not different among experimental groups and was correlated with Oat1 and 3 mRNA expressions. Nevertheless, while insulin-stimulated Oat1 and 3 functions in renal slices were blunted in T2DM rats, they were improved by CGE supplementation. The mechanism of CGE-restored insulin-stimulated Oat1 and 3 functions was clearly shown to be associated with upregulated PKCζ and downregulated PKCα expressions and activations. These findings indicate that CGE has antidiabetic effect and suggest it may prevent diabetic nephropathy through PKCs in a T2DM rat model. Hindawi Publishing Corporation 2015 2015-03-26 /pmc/articles/PMC4391723/ /pubmed/25883984 http://dx.doi.org/10.1155/2015/320167 Text en Copyright © 2015 Chutima Srimaroeng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Srimaroeng, Chutima
Ontawong, Atcharaporn
Saowakon, Naruwan
Vivithanaporn, Pornpun
Pongchaidecha, Anchalee
Amornlerdpison, Doungporn
Soodvilai, Sunhapas
Chatsudthipong, Varanuj
Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy
title Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy
title_full Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy
title_fullStr Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy
title_full_unstemmed Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy
title_short Antidiabetic and Renoprotective Effects of Cladophora glomerata Kützing Extract in Experimental Type 2 Diabetic Rats: A Potential Nutraceutical Product for Diabetic Nephropathy
title_sort antidiabetic and renoprotective effects of cladophora glomerata kützing extract in experimental type 2 diabetic rats: a potential nutraceutical product for diabetic nephropathy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391723/
https://www.ncbi.nlm.nih.gov/pubmed/25883984
http://dx.doi.org/10.1155/2015/320167
work_keys_str_mv AT srimaroengchutima antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy
AT ontawongatcharaporn antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy
AT saowakonnaruwan antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy
AT vivithanapornpornpun antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy
AT pongchaidechaanchalee antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy
AT amornlerdpisondoungporn antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy
AT soodvilaisunhapas antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy
AT chatsudthipongvaranuj antidiabeticandrenoprotectiveeffectsofcladophoraglomeratakutzingextractinexperimentaltype2diabeticratsapotentialnutraceuticalproductfordiabeticnephropathy