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Purification and Characterization of a DNA-Binding Recombinant PREP1:PBX1 Complex

Human PREP1 and PBX1 are homeodomain transcriptional factors, whose biochemical and structural characterization has not yet been fully described. Expression of full-length recombinant PREP1 (47.6 kDa) and PBX1 (46.6 kDa) in E. coli is difficult because of poor yield, high instability and insufficien...

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Detalles Bibliográficos
Autores principales:	Mathiasen, Lisa, Bruckmann, Chiara, Pasqualato, Sebastiano, Blasi, Francesco
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391845/ https://www.ncbi.nlm.nih.gov/pubmed/25856340 http://dx.doi.org/10.1371/journal.pone.0125789

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