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Codon Optimisation Is Key for Pernisine Expression in Escherichia coli

BACKGROUND: Pernisine is an extracellular serine protease from the hyperthermophilic Archaeon Aeropyrum pernix K1. Low yields from the natural host and expression problems in heterologous hosts have limited the potential applications of pernisine in industry. METHODOLOGY/ PRINCIPAL FINDINGS: The cha...

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Detalles Bibliográficos
Autores principales:	Šnajder, Marko, Mihelič, Marko, Turk, Dušan, Ulrih, Nataša Poklar
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4391949/ https://www.ncbi.nlm.nih.gov/pubmed/25856104 http://dx.doi.org/10.1371/journal.pone.0123288

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