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Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer

Background: Golgi phosphoprotein 3 (GOLPH3) is a metastasis-associated gene, however its role in cell proliferation of prostate cancer (PCa) has not yet been elucidated. Methods: The level of expression of GOLPH3 and other genes was examined by quantitative real-time PCR (QPCR) and western blot anal...

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Autores principales: Li, Wenzhi, Guo, Fengfu, Gu, Meng, Wang, Guangjian, He, Xiangfei, Zhou, Juan, Peng, Yubing, Wang, Zhong, Wang, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392050/
https://www.ncbi.nlm.nih.gov/pubmed/25874005
http://dx.doi.org/10.7150/jca.11228
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author Li, Wenzhi
Guo, Fengfu
Gu, Meng
Wang, Guangjian
He, Xiangfei
Zhou, Juan
Peng, Yubing
Wang, Zhong
Wang, Xiang
author_facet Li, Wenzhi
Guo, Fengfu
Gu, Meng
Wang, Guangjian
He, Xiangfei
Zhou, Juan
Peng, Yubing
Wang, Zhong
Wang, Xiang
author_sort Li, Wenzhi
collection PubMed
description Background: Golgi phosphoprotein 3 (GOLPH3) is a metastasis-associated gene, however its role in cell proliferation of prostate cancer (PCa) has not yet been elucidated. Methods: The level of expression of GOLPH3 and other genes was examined by quantitative real-time PCR (QPCR) and western blot analysis. Furthermore, we performed a comprehensive analysis of the expression of GOLPH3 in PCa using a tissue microarray (TMA) and correlated our findings with pathological parameters of PCa. RNA interference (RNAi) was used to silence the expression of GOLPH3 in PC-3 cells and to measure the effects on proliferation and cell cycle using the CCK-8 assay and flow cytometry. Western blots were also employed to assess AKT-mTOR and cell cycle-related proteins. Results: We showed that the expression of GOLPH3 was located at the trans-Golgi membranes in PCa cells. We found that GOLPH3 was expressed in all PCa cells and was significantly higher in two androgen-independent cell lines, DU145 and PC-3. TMA immunohistochemistry showed that GOLPH3 was positive in 64% of cancer tissue samples compared with 20% in normal and 30% in benign samples (P<0.05). In vitro, silencing GOLPH3 expression inhibited cell proliferation and arrested the cell cycle at the G2/M phase. Silencing GOLPH3 also activated P21 expression but suppressed the expression of CDK1/2 and cyclinB1 protein together with the phosphorylation of AKT and mTOR. Conclusions: The expression of the GOLPH3 protein was significantly elevated in PCa. GOLPH3 can promote cell proliferation by enhancing the activity of AKT-mTOR signaling. Altogether, these findings suggest that GOLPH3 play important roles in proliferation and cell cycle regulation in PCa and might serve as promising biomarkers for PCa progression as well as potential therapeutic targets.
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spelling pubmed-43920502015-04-13 Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer Li, Wenzhi Guo, Fengfu Gu, Meng Wang, Guangjian He, Xiangfei Zhou, Juan Peng, Yubing Wang, Zhong Wang, Xiang J Cancer Research Paper Background: Golgi phosphoprotein 3 (GOLPH3) is a metastasis-associated gene, however its role in cell proliferation of prostate cancer (PCa) has not yet been elucidated. Methods: The level of expression of GOLPH3 and other genes was examined by quantitative real-time PCR (QPCR) and western blot analysis. Furthermore, we performed a comprehensive analysis of the expression of GOLPH3 in PCa using a tissue microarray (TMA) and correlated our findings with pathological parameters of PCa. RNA interference (RNAi) was used to silence the expression of GOLPH3 in PC-3 cells and to measure the effects on proliferation and cell cycle using the CCK-8 assay and flow cytometry. Western blots were also employed to assess AKT-mTOR and cell cycle-related proteins. Results: We showed that the expression of GOLPH3 was located at the trans-Golgi membranes in PCa cells. We found that GOLPH3 was expressed in all PCa cells and was significantly higher in two androgen-independent cell lines, DU145 and PC-3. TMA immunohistochemistry showed that GOLPH3 was positive in 64% of cancer tissue samples compared with 20% in normal and 30% in benign samples (P<0.05). In vitro, silencing GOLPH3 expression inhibited cell proliferation and arrested the cell cycle at the G2/M phase. Silencing GOLPH3 also activated P21 expression but suppressed the expression of CDK1/2 and cyclinB1 protein together with the phosphorylation of AKT and mTOR. Conclusions: The expression of the GOLPH3 protein was significantly elevated in PCa. GOLPH3 can promote cell proliferation by enhancing the activity of AKT-mTOR signaling. Altogether, these findings suggest that GOLPH3 play important roles in proliferation and cell cycle regulation in PCa and might serve as promising biomarkers for PCa progression as well as potential therapeutic targets. Ivyspring International Publisher 2015-02-27 /pmc/articles/PMC4392050/ /pubmed/25874005 http://dx.doi.org/10.7150/jca.11228 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Research Paper
Li, Wenzhi
Guo, Fengfu
Gu, Meng
Wang, Guangjian
He, Xiangfei
Zhou, Juan
Peng, Yubing
Wang, Zhong
Wang, Xiang
Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer
title Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer
title_full Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer
title_fullStr Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer
title_full_unstemmed Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer
title_short Increased Expression of GOLPH3 is Associated with the Proliferation of Prostate Cancer
title_sort increased expression of golph3 is associated with the proliferation of prostate cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392050/
https://www.ncbi.nlm.nih.gov/pubmed/25874005
http://dx.doi.org/10.7150/jca.11228
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