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Mitochondrial DNA alteration in obstructive sleep apnea
BACKGROUND: Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respirator...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392628/ https://www.ncbi.nlm.nih.gov/pubmed/25890226 http://dx.doi.org/10.1186/s12931-015-0205-7 |
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author | Lacedonia, Donato Carpagnano, Giovanna E Crisetti, Elisabetta Cotugno, Grazia Palladino, Grazia P Patricelli, Giulia Sabato, Roberto Foschino Barbaro, Maria P |
author_facet | Lacedonia, Donato Carpagnano, Giovanna E Crisetti, Elisabetta Cotugno, Grazia Palladino, Grazia P Patricelli, Giulia Sabato, Roberto Foschino Barbaro, Maria P |
author_sort | Lacedonia, Donato |
collection | PubMed |
description | BACKGROUND: Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respiratory that have their own DNA (MtDNA). The aim of this study was to investigate if the increase of oxidative stress in OSAS patients can induce also MtDNA alterations. METHODS: 46 OSAS patients (age 59.27 ± 11.38; BMI 30.84 ± 3.64; AHI 36.63 ± 24.18) were compared with 36 control subjects (age 54.42 ± 6.63; BMI 29.06 ± 4.7; AHI 3.8 ± 1.10). In blood cells Content of MtDNA and nuclear DNA (nDNA) was measured in OSAS patients by Real Time PCR. The ratio between MtDNA/nDNA was then calculated. Presence of oxidative stress was evaluated by levels of Reactive Oxygen Metabolites (ROMs), measured by diacron reactive oxygen metabolite test (d-ROM test). RESULTS: MtDNA/nDNA was higher in patients with OSAS than in the control group (150.94 ± 49.14 vs 128.96 ± 45.8; p = 0.04), the levels of ROMs were also higher in OSAS subjects (329.71 ± 70.17 vs 226 ± 36.76; p = 0.04) and they were positively correlated with MtDNA/nDNA (R = 0.5, p < 0.01). CONCLUSIONS: In OSAS patients there is a Mitochondrial DNA damage induced by the increase of oxidative stress. Intermittent hypoxia seems to be the main mechanism which leads to this process. |
format | Online Article Text |
id | pubmed-4392628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43926282015-04-11 Mitochondrial DNA alteration in obstructive sleep apnea Lacedonia, Donato Carpagnano, Giovanna E Crisetti, Elisabetta Cotugno, Grazia Palladino, Grazia P Patricelli, Giulia Sabato, Roberto Foschino Barbaro, Maria P Respir Res Research BACKGROUND: Obstructive Sleep Apnea (OSAS) is a disease associated with the increase of cardiovascular risk and it is characterized by repeated episodes of Intermittent Hypoxia (IH) which inducing oxidative stress and systemic inflammation. Mitochondria are cell organelles involved in the respiratory that have their own DNA (MtDNA). The aim of this study was to investigate if the increase of oxidative stress in OSAS patients can induce also MtDNA alterations. METHODS: 46 OSAS patients (age 59.27 ± 11.38; BMI 30.84 ± 3.64; AHI 36.63 ± 24.18) were compared with 36 control subjects (age 54.42 ± 6.63; BMI 29.06 ± 4.7; AHI 3.8 ± 1.10). In blood cells Content of MtDNA and nuclear DNA (nDNA) was measured in OSAS patients by Real Time PCR. The ratio between MtDNA/nDNA was then calculated. Presence of oxidative stress was evaluated by levels of Reactive Oxygen Metabolites (ROMs), measured by diacron reactive oxygen metabolite test (d-ROM test). RESULTS: MtDNA/nDNA was higher in patients with OSAS than in the control group (150.94 ± 49.14 vs 128.96 ± 45.8; p = 0.04), the levels of ROMs were also higher in OSAS subjects (329.71 ± 70.17 vs 226 ± 36.76; p = 0.04) and they were positively correlated with MtDNA/nDNA (R = 0.5, p < 0.01). CONCLUSIONS: In OSAS patients there is a Mitochondrial DNA damage induced by the increase of oxidative stress. Intermittent hypoxia seems to be the main mechanism which leads to this process. BioMed Central 2015-04-07 2015 /pmc/articles/PMC4392628/ /pubmed/25890226 http://dx.doi.org/10.1186/s12931-015-0205-7 Text en © Lacedonia et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Lacedonia, Donato Carpagnano, Giovanna E Crisetti, Elisabetta Cotugno, Grazia Palladino, Grazia P Patricelli, Giulia Sabato, Roberto Foschino Barbaro, Maria P Mitochondrial DNA alteration in obstructive sleep apnea |
title | Mitochondrial DNA alteration in obstructive sleep apnea |
title_full | Mitochondrial DNA alteration in obstructive sleep apnea |
title_fullStr | Mitochondrial DNA alteration in obstructive sleep apnea |
title_full_unstemmed | Mitochondrial DNA alteration in obstructive sleep apnea |
title_short | Mitochondrial DNA alteration in obstructive sleep apnea |
title_sort | mitochondrial dna alteration in obstructive sleep apnea |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392628/ https://www.ncbi.nlm.nih.gov/pubmed/25890226 http://dx.doi.org/10.1186/s12931-015-0205-7 |
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