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An improved protocol to study the plant cell wall proteome
Cell wall proteins were extracted from alfalfa stems according to a three-steps extraction procedure using sequentially CaCl(2), EGTA, and LiCl-complemented buffers. The efficiency of this protocol for extracting cell wall proteins was compared with the two previously published methods optimized for...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392696/ https://www.ncbi.nlm.nih.gov/pubmed/25914713 http://dx.doi.org/10.3389/fpls.2015.00237 |