A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties

A sequence-specific nicking endonuclease from Streptomyces designated as DC13 was purified to near homogeneity. Starting with 30 grams of wet cells, the enzyme was purified by ammonium sulfate fractionation, DEAE cellulose, and phenyl-Sepharose chromatography. The purified protein had a specific act...

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Detalles Bibliográficos
Autores principales: Somyoonsap, Peechapack, Kitpreechavanich, Vichein, Pornbanlualap, Somchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392989/
https://www.ncbi.nlm.nih.gov/pubmed/25937959
http://dx.doi.org/10.1155/2013/287158

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392989/
https://www.ncbi.nlm.nih.gov/pubmed/25937959
http://dx.doi.org/10.1155/2013/287158

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