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A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
A sequence-specific nicking endonuclease from Streptomyces designated as DC13 was purified to near homogeneity. Starting with 30 grams of wet cells, the enzyme was purified by ammonium sulfate fractionation, DEAE cellulose, and phenyl-Sepharose chromatography. The purified protein had a specific act...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Hindawi Publishing Corporation
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4392989/ https://www.ncbi.nlm.nih.gov/pubmed/25937959 http://dx.doi.org/10.1155/2013/287158 |