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MicroRNA-Like Small RNAs Prediction in the Development of Antrodia cinnamomea

Antrodia cinnamomea, a precious, host-specific brown-rot fungus that has been used as a folk medicine in Taiwan for centuries is known to have diverse bioactive compounds with potent pharmaceutical activity. In this study, different fermentation states of A. cinnamomea (wild-type fruiting bodies and...

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Detalles Bibliográficos
Autores principales: Lin, Yan-Liang, Ma, Li-Ting, Lee, Yi-Ru, Lin, Shih-Shun, Wang, Sheng-Yang, Chang, Tun-Tschu, Shaw, Jei-Fu, Li, Wen-Hsiung, Chu, Fang-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393119/
https://www.ncbi.nlm.nih.gov/pubmed/25860872
http://dx.doi.org/10.1371/journal.pone.0123245
Descripción
Sumario:Antrodia cinnamomea, a precious, host-specific brown-rot fungus that has been used as a folk medicine in Taiwan for centuries is known to have diverse bioactive compounds with potent pharmaceutical activity. In this study, different fermentation states of A. cinnamomea (wild-type fruiting bodies and liquid cultured mycelium) were sequenced using the next-generation sequencing (NGS) technique. A 45.58 Mb genome encoding 6,522 predicted genes was obtained. High quality reads were assembled into a total of 13,109 unigenes. Using a previously constructed pipeline to search for microRNAs (miRNAs), we then identified 4 predicted conserved miRNA and 63 novel predicted miRNA-like small RNA (milRNA) candidates. Target prediction revealed several interesting proteins involved in tri-terpenoid synthesis, mating type recognition, chemical or physical sensory protein and transporters predicted to be regulated by the miRNAs and milRNAs.