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Translocation of TRPV2 channel induced by focal administration of mechanical stress

The effect of focal mechanical stress on the localization of TRPV2 was investigated in HT1080 cells, where only mRNA for TRPV2 was detected among members of the TRPV channel family. Mechanical stress was applied by adding negative pressure using a glass pipette. When focal mechanical stress was appl...

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Autores principales: Nagasawa, Masahiro, Kojima, Itaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393204/
https://www.ncbi.nlm.nih.gov/pubmed/25677550
http://dx.doi.org/10.14814/phy2.12296
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author Nagasawa, Masahiro
Kojima, Itaru
author_facet Nagasawa, Masahiro
Kojima, Itaru
author_sort Nagasawa, Masahiro
collection PubMed
description The effect of focal mechanical stress on the localization of TRPV2 was investigated in HT1080 cells, where only mRNA for TRPV2 was detected among members of the TRPV channel family. Mechanical stress was applied by adding negative pressure using a glass pipette. When focal mechanical stress was applied, subplasma membrane Ca(2+) concentration ([Ca(2+)](s)) was increased beneath the pipette, which propagated throughout the cell. The increase in [Ca(2+)](s) was blocked by ruthenium red or by knocking down TRPV2. Elevation of [Ca(2+)](s) was not observed by removal of extracellular Ca(2+), by an addition of a phosphatidylinositol 3-kinase inhibitor LY29034, and by transfection of dominant-negative Rac. In cells expressing GFP-TRPV2 and RFP-Akt, administration of focal mechanical stress induced accumulation of GFP-TRPV2 beneath the pipette. RFP-Akt was also accumulated to the same site. Gadolinium blocked the elevation of [Ca(2+)](s) induced by focal mechanical stress and also attenuated accumulation of TRPV2. When GFP-TRPV1, GFP-TRPV3, GFP-TRPV4, GFP-TRPV5, or GFP-TRPV6 was transfected ectopically in HT1080 cells, only GFP-TRPV4 was accumulated beneath the pipette in response to the focal mechanical stress. These results indicate that TRPV2 translocates to the site receiving a focal mechanical stress and increases [Ca(2+)](s).
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spelling pubmed-43932042015-04-20 Translocation of TRPV2 channel induced by focal administration of mechanical stress Nagasawa, Masahiro Kojima, Itaru Physiol Rep Original Research The effect of focal mechanical stress on the localization of TRPV2 was investigated in HT1080 cells, where only mRNA for TRPV2 was detected among members of the TRPV channel family. Mechanical stress was applied by adding negative pressure using a glass pipette. When focal mechanical stress was applied, subplasma membrane Ca(2+) concentration ([Ca(2+)](s)) was increased beneath the pipette, which propagated throughout the cell. The increase in [Ca(2+)](s) was blocked by ruthenium red or by knocking down TRPV2. Elevation of [Ca(2+)](s) was not observed by removal of extracellular Ca(2+), by an addition of a phosphatidylinositol 3-kinase inhibitor LY29034, and by transfection of dominant-negative Rac. In cells expressing GFP-TRPV2 and RFP-Akt, administration of focal mechanical stress induced accumulation of GFP-TRPV2 beneath the pipette. RFP-Akt was also accumulated to the same site. Gadolinium blocked the elevation of [Ca(2+)](s) induced by focal mechanical stress and also attenuated accumulation of TRPV2. When GFP-TRPV1, GFP-TRPV3, GFP-TRPV4, GFP-TRPV5, or GFP-TRPV6 was transfected ectopically in HT1080 cells, only GFP-TRPV4 was accumulated beneath the pipette in response to the focal mechanical stress. These results indicate that TRPV2 translocates to the site receiving a focal mechanical stress and increases [Ca(2+)](s). BlackWell Publishing Ltd 2015-02-13 /pmc/articles/PMC4393204/ /pubmed/25677550 http://dx.doi.org/10.14814/phy2.12296 Text en © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Nagasawa, Masahiro
Kojima, Itaru
Translocation of TRPV2 channel induced by focal administration of mechanical stress
title Translocation of TRPV2 channel induced by focal administration of mechanical stress
title_full Translocation of TRPV2 channel induced by focal administration of mechanical stress
title_fullStr Translocation of TRPV2 channel induced by focal administration of mechanical stress
title_full_unstemmed Translocation of TRPV2 channel induced by focal administration of mechanical stress
title_short Translocation of TRPV2 channel induced by focal administration of mechanical stress
title_sort translocation of trpv2 channel induced by focal administration of mechanical stress
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393204/
https://www.ncbi.nlm.nih.gov/pubmed/25677550
http://dx.doi.org/10.14814/phy2.12296
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