Potentials and capabilities of the Extracellular Vesicle (EV) Array

Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2...

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Detalles Bibliográficos
Autores principales: Jørgensen, Malene Møller, Bæk, Rikke, Varming, Kim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2015
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393420/
https://www.ncbi.nlm.nih.gov/pubmed/25862471
http://dx.doi.org/10.3402/jev.v4.26048
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author Jørgensen, Malene Møller
Bæk, Rikke
Varming, Kim
author_facet Jørgensen, Malene Møller
Bæk, Rikke
Varming, Kim
author_sort Jørgensen, Malene Møller
collection PubMed
description Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.
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spelling pubmed-43934202015-04-16 Potentials and capabilities of the Extracellular Vesicle (EV) Array Jørgensen, Malene Møller Bæk, Rikke Varming, Kim J Extracell Vesicles Technical Report Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis. Co-Action Publishing 2015-04-08 /pmc/articles/PMC4393420/ /pubmed/25862471 http://dx.doi.org/10.3402/jev.v4.26048 Text en © 2015 Malene Møller Jørgensen et al. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Report
Jørgensen, Malene Møller
Bæk, Rikke
Varming, Kim
Potentials and capabilities of the Extracellular Vesicle (EV) Array
title Potentials and capabilities of the Extracellular Vesicle (EV) Array
title_full Potentials and capabilities of the Extracellular Vesicle (EV) Array
title_fullStr Potentials and capabilities of the Extracellular Vesicle (EV) Array
title_full_unstemmed Potentials and capabilities of the Extracellular Vesicle (EV) Array
title_short Potentials and capabilities of the Extracellular Vesicle (EV) Array
title_sort potentials and capabilities of the extracellular vesicle (ev) array
topic Technical Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393420/
https://www.ncbi.nlm.nih.gov/pubmed/25862471
http://dx.doi.org/10.3402/jev.v4.26048
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AT bækrikke potentialsandcapabilitiesoftheextracellularvesicleevarray
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