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Complexities due to single-stranded RNA during antibody detection of genomic rna:dna hybrids

BACKGROUND: Long genomic R-loops in eukaryotes were first described at the immunoglobulin heavy chain locus switch regions using bisulfite sequencing and functional studies. A mouse monoclonal antibody called S9.6 has been used for immunoprecipitation (IP) to identify R-loops, based on the assumptio...

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Detalles Bibliográficos
Autores principales:	Zhang, Zheng Z, Pannunzio, Nicholas R, Hsieh, Chih-Lin, Yu, Kefei, Lieber, Michael R
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Short Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393563/ https://www.ncbi.nlm.nih.gov/pubmed/25890199 http://dx.doi.org/10.1186/s13104-015-1092-1

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