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Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells

Acute coronary syndromes and acute myocardial infarctions are often related to plaque rupture and the formation of thrombi at the site of the rupture. We examined fresh coronary thrombectomy specimens from patients with acute coronary syndromes and assessed their structure and cellularity. The throm...

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Autores principales: Jovin, Ion S, Lei, Li, Huang, Yan, Hao, Zhengrong, Curtis, Jeptha P, Brennan, Joseph J, Remetz, Michael S, Setaro, John F, Pfau, Steven E, Howes, Christopher J, Clancy, Jude F, Cabin, Henry S, Cleman, Michael W, Giordano, Frank J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393730/
https://www.ncbi.nlm.nih.gov/pubmed/22947374
http://dx.doi.org/10.1111/j.1582-4934.2012.01629.x
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author Jovin, Ion S
Lei, Li
Huang, Yan
Hao, Zhengrong
Curtis, Jeptha P
Brennan, Joseph J
Remetz, Michael S
Setaro, John F
Pfau, Steven E
Howes, Christopher J
Clancy, Jude F
Cabin, Henry S
Cleman, Michael W
Giordano, Frank J
author_facet Jovin, Ion S
Lei, Li
Huang, Yan
Hao, Zhengrong
Curtis, Jeptha P
Brennan, Joseph J
Remetz, Michael S
Setaro, John F
Pfau, Steven E
Howes, Christopher J
Clancy, Jude F
Cabin, Henry S
Cleman, Michael W
Giordano, Frank J
author_sort Jovin, Ion S
collection PubMed
description Acute coronary syndromes and acute myocardial infarctions are often related to plaque rupture and the formation of thrombi at the site of the rupture. We examined fresh coronary thrombectomy specimens from patients with acute coronary syndromes and assessed their structure and cellularity. The thrombectomy specimens consisted of platelets, erythrocytes and inflammatory cells. Several specimens contained multiple cholesterol crystals. Culture of thrombectomy specimens yielded cells growing in various patterns depending on the culture medium used. Culture in serum-free stem cell enrichment medium yielded cells with features of endothelial progenitor cells which survived in culture for a year. Immunohistochemical analysis of the thrombi revealed cells positive for CD34, cells positive for CD15 and cells positive for desmin in situ, whereas cultured cell from thrombi was desmin positive but pancytokeratin negative. Cells cultured in endothelial cell medium were von Willebrand factor positive. The content of coronary thrombectomy specimens is heterogeneous and consists of blood cells but also possibly cells from the vascular wall and cholesterol crystals. The culture of cells contained in the specimens yielded multiplying cells, some of which demonstrated features of haematopoietic progenitor cells and which differentiated into various cell-types.
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spelling pubmed-43937302015-04-13 Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells Jovin, Ion S Lei, Li Huang, Yan Hao, Zhengrong Curtis, Jeptha P Brennan, Joseph J Remetz, Michael S Setaro, John F Pfau, Steven E Howes, Christopher J Clancy, Jude F Cabin, Henry S Cleman, Michael W Giordano, Frank J J Cell Mol Med Original Articles Acute coronary syndromes and acute myocardial infarctions are often related to plaque rupture and the formation of thrombi at the site of the rupture. We examined fresh coronary thrombectomy specimens from patients with acute coronary syndromes and assessed their structure and cellularity. The thrombectomy specimens consisted of platelets, erythrocytes and inflammatory cells. Several specimens contained multiple cholesterol crystals. Culture of thrombectomy specimens yielded cells growing in various patterns depending on the culture medium used. Culture in serum-free stem cell enrichment medium yielded cells with features of endothelial progenitor cells which survived in culture for a year. Immunohistochemical analysis of the thrombi revealed cells positive for CD34, cells positive for CD15 and cells positive for desmin in situ, whereas cultured cell from thrombi was desmin positive but pancytokeratin negative. Cells cultured in endothelial cell medium were von Willebrand factor positive. The content of coronary thrombectomy specimens is heterogeneous and consists of blood cells but also possibly cells from the vascular wall and cholesterol crystals. The culture of cells contained in the specimens yielded multiplying cells, some of which demonstrated features of haematopoietic progenitor cells and which differentiated into various cell-types. BlackWell Publishing Ltd 2012-12 2012-12-13 /pmc/articles/PMC4393730/ /pubmed/22947374 http://dx.doi.org/10.1111/j.1582-4934.2012.01629.x Text en © 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Original Articles
Jovin, Ion S
Lei, Li
Huang, Yan
Hao, Zhengrong
Curtis, Jeptha P
Brennan, Joseph J
Remetz, Michael S
Setaro, John F
Pfau, Steven E
Howes, Christopher J
Clancy, Jude F
Cabin, Henry S
Cleman, Michael W
Giordano, Frank J
Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
title Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
title_full Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
title_fullStr Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
title_full_unstemmed Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
title_short Cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
title_sort cellularity and structure of fresh human coronary thrombectomy specimens; presence of cells with markers of progenitor cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393730/
https://www.ncbi.nlm.nih.gov/pubmed/22947374
http://dx.doi.org/10.1111/j.1582-4934.2012.01629.x
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