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Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts

BACKGROUND: Plants colonized terrestrial environments approximately 480 million years ago and have contributed significantly to the diversification of life on Earth. Phylogenetic analyses position a subset of charophyte algae as the sister group to land plants, and distinguish two land plant groups...

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Autores principales: Szövényi, Péter, Frangedakis, Eftychios, Ricca, Mariana, Quandt, Dietmar, Wicke, Susann, Langdale, Jane A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393856/
https://www.ncbi.nlm.nih.gov/pubmed/25886741
http://dx.doi.org/10.1186/s12870-015-0481-x
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author Szövényi, Péter
Frangedakis, Eftychios
Ricca, Mariana
Quandt, Dietmar
Wicke, Susann
Langdale, Jane A
author_facet Szövényi, Péter
Frangedakis, Eftychios
Ricca, Mariana
Quandt, Dietmar
Wicke, Susann
Langdale, Jane A
author_sort Szövényi, Péter
collection PubMed
description BACKGROUND: Plants colonized terrestrial environments approximately 480 million years ago and have contributed significantly to the diversification of life on Earth. Phylogenetic analyses position a subset of charophyte algae as the sister group to land plants, and distinguish two land plant groups that diverged around 450 million years ago – the bryophytes and the vascular plants. Relationships between liverworts, mosses hornworts and vascular plants have proven difficult to resolve, and as such it is not clear which bryophyte lineage is the sister group to all other land plants and which is the sister to vascular plants. The lack of comparative molecular studies in representatives of all three lineages exacerbates this uncertainty. Such comparisons can be made between mosses and liverworts because representative model organisms are well established in these two bryophyte lineages. To date, however, a model hornwort species has not been available. RESULTS: Here we report the establishment of Anthoceros agrestis as a model hornwort species for laboratory experiments. Axenic culture conditions for maintenance and vegetative propagation have been determined, and treatments for the induction of sexual reproduction and sporophyte development have been established. In addition, protocols have been developed for the extraction of DNA and RNA that is of a quality suitable for molecular analyses. Analysis of haploid-derived genome sequence data of two A. agrestis isolates revealed single nucleotide polymorphisms at multiple loci, and thus these two strains are suitable starting material for classical genetic and mapping experiments. CONCLUSIONS: Methods and resources have been developed to enable A. agrestis to be used as a model species for developmental, molecular, genomic, and genetic studies. This advance provides an unprecedented opportunity to investigate the biology of hornworts.
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spelling pubmed-43938562015-04-13 Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts Szövényi, Péter Frangedakis, Eftychios Ricca, Mariana Quandt, Dietmar Wicke, Susann Langdale, Jane A BMC Plant Biol Methodology Article BACKGROUND: Plants colonized terrestrial environments approximately 480 million years ago and have contributed significantly to the diversification of life on Earth. Phylogenetic analyses position a subset of charophyte algae as the sister group to land plants, and distinguish two land plant groups that diverged around 450 million years ago – the bryophytes and the vascular plants. Relationships between liverworts, mosses hornworts and vascular plants have proven difficult to resolve, and as such it is not clear which bryophyte lineage is the sister group to all other land plants and which is the sister to vascular plants. The lack of comparative molecular studies in representatives of all three lineages exacerbates this uncertainty. Such comparisons can be made between mosses and liverworts because representative model organisms are well established in these two bryophyte lineages. To date, however, a model hornwort species has not been available. RESULTS: Here we report the establishment of Anthoceros agrestis as a model hornwort species for laboratory experiments. Axenic culture conditions for maintenance and vegetative propagation have been determined, and treatments for the induction of sexual reproduction and sporophyte development have been established. In addition, protocols have been developed for the extraction of DNA and RNA that is of a quality suitable for molecular analyses. Analysis of haploid-derived genome sequence data of two A. agrestis isolates revealed single nucleotide polymorphisms at multiple loci, and thus these two strains are suitable starting material for classical genetic and mapping experiments. CONCLUSIONS: Methods and resources have been developed to enable A. agrestis to be used as a model species for developmental, molecular, genomic, and genetic studies. This advance provides an unprecedented opportunity to investigate the biology of hornworts. BioMed Central 2015-04-09 /pmc/articles/PMC4393856/ /pubmed/25886741 http://dx.doi.org/10.1186/s12870-015-0481-x Text en © Szövényi et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology Article
Szövényi, Péter
Frangedakis, Eftychios
Ricca, Mariana
Quandt, Dietmar
Wicke, Susann
Langdale, Jane A
Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts
title Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts
title_full Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts
title_fullStr Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts
title_full_unstemmed Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts
title_short Establishment of Anthoceros agrestis as a model species for studying the biology of hornworts
title_sort establishment of anthoceros agrestis as a model species for studying the biology of hornworts
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393856/
https://www.ncbi.nlm.nih.gov/pubmed/25886741
http://dx.doi.org/10.1186/s12870-015-0481-x
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