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Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus

Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases...

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Autores principales: Wang, Yun-Peng, Wei, Zheng-Yi, Zhang, Yu-Ying, Lin, Chun-Jing, Zhong, Xiao-Fang, Wang, Yue-Lin, Ma, Jing-Yong, Ma, Jian, Xing, Shao-Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394439/
https://www.ncbi.nlm.nih.gov/pubmed/25739079
http://dx.doi.org/10.3390/ijms16034628
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author Wang, Yun-Peng
Wei, Zheng-Yi
Zhang, Yu-Ying
Lin, Chun-Jing
Zhong, Xiao-Fang
Wang, Yue-Lin
Ma, Jing-Yong
Ma, Jian
Xing, Shao-Chen
author_facet Wang, Yun-Peng
Wei, Zheng-Yi
Zhang, Yu-Ying
Lin, Chun-Jing
Zhong, Xiao-Fang
Wang, Yue-Lin
Ma, Jing-Yong
Ma, Jian
Xing, Shao-Chen
author_sort Wang, Yun-Peng
collection PubMed
description Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 °C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast.
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spelling pubmed-43944392015-05-21 Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus Wang, Yun-Peng Wei, Zheng-Yi Zhang, Yu-Ying Lin, Chun-Jing Zhong, Xiao-Fang Wang, Yue-Lin Ma, Jing-Yong Ma, Jian Xing, Shao-Chen Int J Mol Sci Article Rice blast is a major destructive fungal disease that poses a serious threat to rice production and the improvement of blast resistance is critical to rice breeding. The antimicrobial peptide MSI-99 has been suggested as an antimicrobial peptide conferring resistance to bacterial and fungal diseases. Here, a vector harboring the MSI-99 gene was constructed and introduced into the tobacco chloroplast genome via particle bombardment. Transformed plants were obtained and verified to be homoplastomic by PCR and Southern hybridization. In planta assays demonstrated that the transgenic tobacco plants displayed an enhanced resistance to the fungal disease. The evaluation of the antimicrobial activity revealed that the crude protein extracts from the transgenic plants manifested an antimicrobial activity against E. coli, even after incubation at 120 °C for 20 min, indicating significant heat stability of MSI-99. More importantly, the MSI-99-containing protein extracts were firstly proved in vitro and in vivo to display significant suppressive effects on two rice blast isolates. These findings provide a strong basis for the development of new biopesticides to combat rice blast. MDPI 2015-03-02 /pmc/articles/PMC4394439/ /pubmed/25739079 http://dx.doi.org/10.3390/ijms16034628 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yun-Peng
Wei, Zheng-Yi
Zhang, Yu-Ying
Lin, Chun-Jing
Zhong, Xiao-Fang
Wang, Yue-Lin
Ma, Jing-Yong
Ma, Jian
Xing, Shao-Chen
Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus
title Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus
title_full Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus
title_fullStr Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus
title_full_unstemmed Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus
title_short Chloroplast-Expressed MSI-99 in Tobacco Improves Disease Resistance and Displays Inhibitory Effect against Rice Blast Fungus
title_sort chloroplast-expressed msi-99 in tobacco improves disease resistance and displays inhibitory effect against rice blast fungus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394439/
https://www.ncbi.nlm.nih.gov/pubmed/25739079
http://dx.doi.org/10.3390/ijms16034628
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