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A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic regeneration method based on the use of...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394441/ https://www.ncbi.nlm.nih.gov/pubmed/25739081 http://dx.doi.org/10.3390/ijms16034666 |
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author | Ferroni, Letizia Gardin, Chiara Sivolella, Stefano Brunello, Giulia Berengo, Mario Piattelli, Adriano Bressan, Eriberto Zavan, Barbara |
author_facet | Ferroni, Letizia Gardin, Chiara Sivolella, Stefano Brunello, Giulia Berengo, Mario Piattelli, Adriano Bressan, Eriberto Zavan, Barbara |
author_sort | Ferroni, Letizia |
collection | PubMed |
description | Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic regeneration method based on the use of a tridimensional (3D) hyaluronan scaffold and human dental pulp stem cells (DPSCs) to produce a functional dental pulp-like tissue in vitro. An enriched population of DPSCs was seeded onto hyaluronan-based non-woven meshes in the presence of differentiation factors to induce the commitment of stem cells to neuronal, glial, endothelial and osteogenic phenotypes. In vitro experiments, among which were gene expression profiling and immunofluorescence (IF) staining, proved the commitment of DPSCs to the main components of dental pulp tissue. In particular, the hyaluronan-DPSCs construct showed a dental pulp-like morphology consisting of several specialized cells growing inside the hyaluronan fibers. Furthermore, these constructs were implanted into rat calvarial critical-size defects. Histological analyses and gene expression profiling performed on hyaluronan-DPSCs grafts showed the regeneration of osteodentin-like tissue. Altogether, these data suggest the regenerative potential of the hyaluronan-DPSC engineered tissue. |
format | Online Article Text |
id | pubmed-4394441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-43944412015-05-21 A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue Ferroni, Letizia Gardin, Chiara Sivolella, Stefano Brunello, Giulia Berengo, Mario Piattelli, Adriano Bressan, Eriberto Zavan, Barbara Int J Mol Sci Article Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic regeneration method based on the use of a tridimensional (3D) hyaluronan scaffold and human dental pulp stem cells (DPSCs) to produce a functional dental pulp-like tissue in vitro. An enriched population of DPSCs was seeded onto hyaluronan-based non-woven meshes in the presence of differentiation factors to induce the commitment of stem cells to neuronal, glial, endothelial and osteogenic phenotypes. In vitro experiments, among which were gene expression profiling and immunofluorescence (IF) staining, proved the commitment of DPSCs to the main components of dental pulp tissue. In particular, the hyaluronan-DPSCs construct showed a dental pulp-like morphology consisting of several specialized cells growing inside the hyaluronan fibers. Furthermore, these constructs were implanted into rat calvarial critical-size defects. Histological analyses and gene expression profiling performed on hyaluronan-DPSCs grafts showed the regeneration of osteodentin-like tissue. Altogether, these data suggest the regenerative potential of the hyaluronan-DPSC engineered tissue. MDPI 2015-03-02 /pmc/articles/PMC4394441/ /pubmed/25739081 http://dx.doi.org/10.3390/ijms16034666 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferroni, Letizia Gardin, Chiara Sivolella, Stefano Brunello, Giulia Berengo, Mario Piattelli, Adriano Bressan, Eriberto Zavan, Barbara A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue |
title | A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue |
title_full | A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue |
title_fullStr | A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue |
title_full_unstemmed | A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue |
title_short | A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue |
title_sort | hyaluronan-based scaffold for the in vitro construction of dental pulp-like tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394441/ https://www.ncbi.nlm.nih.gov/pubmed/25739081 http://dx.doi.org/10.3390/ijms16034666 |
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