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A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue

Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic regeneration method based on the use of...

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Autores principales: Ferroni, Letizia, Gardin, Chiara, Sivolella, Stefano, Brunello, Giulia, Berengo, Mario, Piattelli, Adriano, Bressan, Eriberto, Zavan, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394441/
https://www.ncbi.nlm.nih.gov/pubmed/25739081
http://dx.doi.org/10.3390/ijms16034666
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author Ferroni, Letizia
Gardin, Chiara
Sivolella, Stefano
Brunello, Giulia
Berengo, Mario
Piattelli, Adriano
Bressan, Eriberto
Zavan, Barbara
author_facet Ferroni, Letizia
Gardin, Chiara
Sivolella, Stefano
Brunello, Giulia
Berengo, Mario
Piattelli, Adriano
Bressan, Eriberto
Zavan, Barbara
author_sort Ferroni, Letizia
collection PubMed
description Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic regeneration method based on the use of a tridimensional (3D) hyaluronan scaffold and human dental pulp stem cells (DPSCs) to produce a functional dental pulp-like tissue in vitro. An enriched population of DPSCs was seeded onto hyaluronan-based non-woven meshes in the presence of differentiation factors to induce the commitment of stem cells to neuronal, glial, endothelial and osteogenic phenotypes. In vitro experiments, among which were gene expression profiling and immunofluorescence (IF) staining, proved the commitment of DPSCs to the main components of dental pulp tissue. In particular, the hyaluronan-DPSCs construct showed a dental pulp-like morphology consisting of several specialized cells growing inside the hyaluronan fibers. Furthermore, these constructs were implanted into rat calvarial critical-size defects. Histological analyses and gene expression profiling performed on hyaluronan-DPSCs grafts showed the regeneration of osteodentin-like tissue. Altogether, these data suggest the regenerative potential of the hyaluronan-DPSC engineered tissue.
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spelling pubmed-43944412015-05-21 A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue Ferroni, Letizia Gardin, Chiara Sivolella, Stefano Brunello, Giulia Berengo, Mario Piattelli, Adriano Bressan, Eriberto Zavan, Barbara Int J Mol Sci Article Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic regeneration method based on the use of a tridimensional (3D) hyaluronan scaffold and human dental pulp stem cells (DPSCs) to produce a functional dental pulp-like tissue in vitro. An enriched population of DPSCs was seeded onto hyaluronan-based non-woven meshes in the presence of differentiation factors to induce the commitment of stem cells to neuronal, glial, endothelial and osteogenic phenotypes. In vitro experiments, among which were gene expression profiling and immunofluorescence (IF) staining, proved the commitment of DPSCs to the main components of dental pulp tissue. In particular, the hyaluronan-DPSCs construct showed a dental pulp-like morphology consisting of several specialized cells growing inside the hyaluronan fibers. Furthermore, these constructs were implanted into rat calvarial critical-size defects. Histological analyses and gene expression profiling performed on hyaluronan-DPSCs grafts showed the regeneration of osteodentin-like tissue. Altogether, these data suggest the regenerative potential of the hyaluronan-DPSC engineered tissue. MDPI 2015-03-02 /pmc/articles/PMC4394441/ /pubmed/25739081 http://dx.doi.org/10.3390/ijms16034666 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ferroni, Letizia
Gardin, Chiara
Sivolella, Stefano
Brunello, Giulia
Berengo, Mario
Piattelli, Adriano
Bressan, Eriberto
Zavan, Barbara
A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
title A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
title_full A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
title_fullStr A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
title_full_unstemmed A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
title_short A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue
title_sort hyaluronan-based scaffold for the in vitro construction of dental pulp-like tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394441/
https://www.ncbi.nlm.nih.gov/pubmed/25739081
http://dx.doi.org/10.3390/ijms16034666
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