Cargando…

Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro

Colorectal cancer, breast cancer and skin cancer are commonly-reported cancer types in the U.S. Although radiation and chemotherapy are routinely used to treat cancer, they produce side effects in patients. Additionally, resistance to chemotherapeutic drugs has been noticed in cancers. Thus, there i...

Descripción completa

Detalles Bibliográficos
Autores principales: Narayanan, Amoolya, Ananda Baskaran, Sangeetha, Amalaradjou, Mary Anne Roshni, Venkitanarayanan, Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394462/
https://www.ncbi.nlm.nih.gov/pubmed/25749477
http://dx.doi.org/10.3390/ijms16035014
_version_ 1782366289750130688
author Narayanan, Amoolya
Ananda Baskaran, Sangeetha
Amalaradjou, Mary Anne Roshni
Venkitanarayanan, Kumar
author_facet Narayanan, Amoolya
Ananda Baskaran, Sangeetha
Amalaradjou, Mary Anne Roshni
Venkitanarayanan, Kumar
author_sort Narayanan, Amoolya
collection PubMed
description Colorectal cancer, breast cancer and skin cancer are commonly-reported cancer types in the U.S. Although radiation and chemotherapy are routinely used to treat cancer, they produce side effects in patients. Additionally, resistance to chemotherapeutic drugs has been noticed in cancers. Thus, there is a need for effective and safe bioprophylactics and biotherapeutics in cancer therapy. The medicinal value of goat milk has been recognized for centuries and is primarily attributed to three fatty acids, namely capric, caprylic and caproic acids. This research investigates the anticancer property of these fatty acids on human colorectal, skin and mammary gland cancer cells. The cancer cells were treated with various concentrations of fatty acids for 48 h, and cell viability was monitored by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. Additionally, real-time quantitative PCR (RT-qPCR) was performed to elucidate the potential anti-cancer mechanisms of the three fatty acids under investigation. Capric, caprylic and caproic acids reduced cancer cell viability by 70% to 90% (p < 0.05) compared to controls. RT-qPCR data indicated that these natural molecules produced anticancer effects by down-regulating cell cycle regulatory genes and up-regulating genes involved in apoptosis. Future research will validate the anticancer effect of these fatty acids in an appropriate in vivo model.
format Online
Article
Text
id pubmed-4394462
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-43944622015-05-21 Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro Narayanan, Amoolya Ananda Baskaran, Sangeetha Amalaradjou, Mary Anne Roshni Venkitanarayanan, Kumar Int J Mol Sci Article Colorectal cancer, breast cancer and skin cancer are commonly-reported cancer types in the U.S. Although radiation and chemotherapy are routinely used to treat cancer, they produce side effects in patients. Additionally, resistance to chemotherapeutic drugs has been noticed in cancers. Thus, there is a need for effective and safe bioprophylactics and biotherapeutics in cancer therapy. The medicinal value of goat milk has been recognized for centuries and is primarily attributed to three fatty acids, namely capric, caprylic and caproic acids. This research investigates the anticancer property of these fatty acids on human colorectal, skin and mammary gland cancer cells. The cancer cells were treated with various concentrations of fatty acids for 48 h, and cell viability was monitored by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. Additionally, real-time quantitative PCR (RT-qPCR) was performed to elucidate the potential anti-cancer mechanisms of the three fatty acids under investigation. Capric, caprylic and caproic acids reduced cancer cell viability by 70% to 90% (p < 0.05) compared to controls. RT-qPCR data indicated that these natural molecules produced anticancer effects by down-regulating cell cycle regulatory genes and up-regulating genes involved in apoptosis. Future research will validate the anticancer effect of these fatty acids in an appropriate in vivo model. MDPI 2015-03-05 /pmc/articles/PMC4394462/ /pubmed/25749477 http://dx.doi.org/10.3390/ijms16035014 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Narayanan, Amoolya
Ananda Baskaran, Sangeetha
Amalaradjou, Mary Anne Roshni
Venkitanarayanan, Kumar
Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro
title Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro
title_full Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro
title_fullStr Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro
title_full_unstemmed Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro
title_short Anticarcinogenic Properties of Medium Chain Fatty Acids on Human Colorectal, Skin and Breast Cancer Cells in Vitro
title_sort anticarcinogenic properties of medium chain fatty acids on human colorectal, skin and breast cancer cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394462/
https://www.ncbi.nlm.nih.gov/pubmed/25749477
http://dx.doi.org/10.3390/ijms16035014
work_keys_str_mv AT narayananamoolya anticarcinogenicpropertiesofmediumchainfattyacidsonhumancolorectalskinandbreastcancercellsinvitro
AT anandabaskaransangeetha anticarcinogenicpropertiesofmediumchainfattyacidsonhumancolorectalskinandbreastcancercellsinvitro
AT amalaradjoumaryanneroshni anticarcinogenicpropertiesofmediumchainfattyacidsonhumancolorectalskinandbreastcancercellsinvitro
AT venkitanarayanankumar anticarcinogenicpropertiesofmediumchainfattyacidsonhumancolorectalskinandbreastcancercellsinvitro