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Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells
Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394510/ https://www.ncbi.nlm.nih.gov/pubmed/25782154 http://dx.doi.org/10.3390/ijms16035864 |
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author | Ren, Chun-E Zhu, Xueqiong Li, Jinping Lyle, Christian Dowdy, Sean Podratz, Karl C. Byck, David Chen, Hai-Bin Jiang, Shi-Wen |
author_facet | Ren, Chun-E Zhu, Xueqiong Li, Jinping Lyle, Christian Dowdy, Sean Podratz, Karl C. Byck, David Chen, Hai-Bin Jiang, Shi-Wen |
author_sort | Ren, Chun-E |
collection | PubMed |
description | Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stromal cells from normal human endometrium and investigated the morphological and transcriptional changes induced by estrogen, progesterone and tamoxifen. We demonstrated that stromal cells express appreciable levels of estrogen and progesterone receptors and undergo different morphological changes upon hormonal stimulation. Microarray analysis indicated that both estrogen and progesterone induced dramatic alterations in a variety of genes associated with cell structure, transcription, cell cycle, and signaling. However, divergent patterns of changes, and in some genes opposite effects, were observed for the two hormones. A large number of genes are identified as novel targets for hormonal regulation. These hormone-responsive genes may be involved in normal uterine function and the development of endometrial malignancies. |
format | Online Article Text |
id | pubmed-4394510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-43945102015-05-21 Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells Ren, Chun-E Zhu, Xueqiong Li, Jinping Lyle, Christian Dowdy, Sean Podratz, Karl C. Byck, David Chen, Hai-Bin Jiang, Shi-Wen Int J Mol Sci Article Epithelial stromal cells represent a major cellular component of human uterine endometrium that is subject to tight hormonal regulation. Through cell-cell contacts and/or paracrine mechanisms, stromal cells play a significant role in the malignant transformation of epithelial cells. We isolated stromal cells from normal human endometrium and investigated the morphological and transcriptional changes induced by estrogen, progesterone and tamoxifen. We demonstrated that stromal cells express appreciable levels of estrogen and progesterone receptors and undergo different morphological changes upon hormonal stimulation. Microarray analysis indicated that both estrogen and progesterone induced dramatic alterations in a variety of genes associated with cell structure, transcription, cell cycle, and signaling. However, divergent patterns of changes, and in some genes opposite effects, were observed for the two hormones. A large number of genes are identified as novel targets for hormonal regulation. These hormone-responsive genes may be involved in normal uterine function and the development of endometrial malignancies. MDPI 2015-03-13 /pmc/articles/PMC4394510/ /pubmed/25782154 http://dx.doi.org/10.3390/ijms16035864 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ren, Chun-E Zhu, Xueqiong Li, Jinping Lyle, Christian Dowdy, Sean Podratz, Karl C. Byck, David Chen, Hai-Bin Jiang, Shi-Wen Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells |
title | Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells |
title_full | Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells |
title_fullStr | Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells |
title_full_unstemmed | Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells |
title_short | Microarray Analysis on Gene Regulation by Estrogen, Progesterone and Tamoxifen in Human Endometrial Stromal Cells |
title_sort | microarray analysis on gene regulation by estrogen, progesterone and tamoxifen in human endometrial stromal cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4394510/ https://www.ncbi.nlm.nih.gov/pubmed/25782154 http://dx.doi.org/10.3390/ijms16035864 |
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